eprintid: 25471
rev_number: 12
eprint_status: archive
userid: 1589
dir: disk0/00/02/54/71
datestamp: 2018-10-18 13:09:22
lastmod: 2024-06-30 21:53:31
status_changed: 2018-10-18 13:09:22
type: article
metadata_visibility: show
creators_name: Freis, Alexander
creators_name: Schlegel, Janina
creators_name: Daniel, Volker
creators_name: Jauckus, Julia
creators_name: Strowitzki, Thomas
creators_name: Germeyer, Ariane
title: Cytokines in relation to hCG are significantly altered in asymptomatic women with miscarriage – a pilot study
subjects: ddc-610
divisions: i-910400
divisions: i-911600
abstract: Background: Spontaneous abortion is one of the most common complications in early pregnancy. A preventive test to identify women who will experience a miscarriage, even before first symptoms occur, is not established. Activation of maternal immunological tolerance seems to be essential for early fetal development and various cytokines have been described in different stages of pregnancy. Therefore, we aimed to investigate if chemokine levels at the time of pregnancy testing relative to human Choriogonadotropin (hCG) are altered in patients who will experience a miscarriage in this pregnancy.   

Methods: We obtained blood samples from 39 women. Dependent on the follow-up, patients with a positive pregnancy test were subsequently divided in two groups: ongoing pregnancy (n = 22) and miscarriage (n = 17) in this pregnancy. Immunological and endocrine profiling of maternal plasma at the time of pregnancy testing (5th week of gestation) was performed for each group at the time of pregnancy test using Multiplex and ELISA analysis. 

Results: hCG was significantly decreased in patients with abortion whereas levels of IL-1ra, MIP-1a and TNF-alpha were significantly increased. GCSF/ IL-1ra-ratio was 1.66-fold increased in patients with ongoing pregnancy. TGF-beta /MIP1a-ratio was significantly 3.45-times higher in patients with miscarriage. Comparing patients with ongoing pregnancy to patients experiencing a miscarriage, we could demonstrate significant alterations of the ratios MIP1a/hCG, IL-1ra/hCG, TNFalpha/hCG, MCP1/hCG, IL-6/hCG, TPO/hCG and TGF-beta1/hCG. The strongest effects were seen for the ratio MIP1a/hCG, IL-1ra/hCG and TNFalpha/hCG.

Conclusions: We have shown that cytokines in relation to hCG after 4 weeks of gestation are significantly altered in women with miscarriage, promising potential as a prognostic biomarker.
date: 2018
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1656917823
own_urn: urn:nbn:de:bsz:16-heidok-254710
language: eng
bibsort: FREISALEXACYTOKINESI2018
full_text_status: public
publication: Reproductive Biology and Endocrinology
volume: 16
number: 93
place_of_pub: London
pagerange: 1-7
issn: 1477-7827
citation:   Freis, Alexander ; Schlegel, Janina ; Daniel, Volker ; Jauckus, Julia ; Strowitzki, Thomas ; Germeyer, Ariane  (2018) Cytokines in relation to hCG are significantly altered in asymptomatic women with miscarriage – a pilot study.  Reproductive Biology and Endocrinology, 16 (93).  pp. 1-7.  ISSN 1477-7827     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/25471/1/12958_2018_Article_411.pdf