title: The methylome of the marbled crayfish links gene body methylation to stable expression of poorly accessible genes
creator: Gatzmann, Fanny
creator: Falckenhayn, Cassandra
creator: Gutekunst, Julian
creator: Hanna, Katharina
creator: Raddatz, Günter
creator: Carneiro, Vitor Coutinho
creator: Lyko, Frank
subject: ddc-610
subject: 610 Medical sciences Medicine
description: Background: The parthenogenetic marbled crayfish (Procambarus virginalis) is a novel species that has rapidly invaded and colonized various different habitats. Adaptation to different environments appears to be independent of the selection of genetic variants, but epigenetic programming of the marbled crayfish genome remains to be understood.    Results: Here, we provide a comprehensive analysis of DNA methylation in marbled crayfish. Whole-genome bisulfite sequencing of multiple replicates and different tissues revealed a methylation pattern that is characterized by gene body methylation of housekeeping genes. Interestingly, this pattern was largely tissue invariant, suggesting a function that is unrelated to cell fate specification. Indeed, integrative analysis of DNA methylation, chromatin accessibility and mRNA expression patterns revealed that gene body methylation correlated with limited chromatin accessibility and stable gene expression, while low-methylated genes often resided in chromatin with higher accessibility and showed increased expression variation. Interestingly, marbled crayfish also showed reduced gene body methylation and higher gene expression variability when compared with their noninvasive mother species, Procambarus fallax.    Conclusions: Our results provide novel insights into invertebrate gene body methylation and its potential role in adaptive gene regulation.
publisher: BioMed Central
date: 2018
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/25475/1/13072_2018_Article_229.pdf
identifier: DOI:
identifier: urn:nbn:de:bsz:16-heidok-254758
identifier:   Gatzmann, Fanny ; Falckenhayn, Cassandra ; Gutekunst, Julian ; Hanna, Katharina ; Raddatz, Günter ; Carneiro, Vitor Coutinho ; Lyko, Frank  (2018) The methylome of the marbled crayfish links gene body methylation to stable expression of poorly accessible genes.  Epigenetics & Chromatin, 11 (57).  pp. 1-12.  ISSN 1756-8935     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/25475/
rights: info:eu-repo/semantics/openAccess
rights: Please see front page of the work (Sorry, Dublin Core plugin does not recognise license id)
language: eng