…
comment: The repository administrator has not yet configured an RDF license.
primary topic: Quantifying the Impact of Cell-to-Cell Transmission on Viral Spread

Quantifying the Impact of Cell-to-Cell Transmission on Viral Spread
type: Article
abstract: Over time, viruses have evolved several strategies to infect host cells, which can be roughly categorized into two distinct modes of viral transmission, namely cell-free and cell-to-cell. Various studies have opted for the latter to play the dominant role in infections with human immunodeficiency virus (HIV) and hepatitis C virus (HCV). How to quantify the exact contribution of each transmission mode to the infection dynamics, without biasing results due to the experimental protocol, remains to be answered to allow for more efficient drug and vaccine development. Previously, mathematical models based on mass-action kinetics were fitted to bulk measurements obtained from in vitro cell cultures cultivated in aqueous media. This combination of experiment and modeling is very specific and might not be appropriate to describe other situations such as in vivo studies. To allow generalization, the effect of modeling strategy and experimental protocol on the predicted contributions of cell-to-cell spread to the infection dynamics should be studied in more detail. In this thesis, I therefore analyzed possible consequences of varying extracellular microenvironments and types of target cells on the different transmission modes during an infection and how certain modeling strategies affect predictions. In a first step, I studied the impact of varying extracellular microenvironments on in vitro spread of HIV. To this end, I developed a detailed mass-action kinetics model combining experiments and mathematical modeling to describe HIV infection dynamics in aqueous media and two types of collagen. My results show that for HIV infection of motile target cells the contribution of transmission modes varies dependent on the environment. Next, I moved from motile to stationary cells analyzing cell-to-cell transmission of HCV among immobile hepatocytes. To account for the local nature of cell-to-cell spread in stationary target cells, I developed an agent-based model, which combines det…
date: 2019
author list: authors
type: EPrint
type: DoctoralThesisEPrint
is part of: repository
has document: Quantifying the Impact of Cell-to-Cell Transmission on Viral Spread (PDF)
has document: Quantifying the Impact of Cell-to-Cell Transmission on Viral Spread (Other)
subject: 500 Naturwissenschaften und Mathematik
subject: 570 Biowissenschaften, Biologie

Quantifying the Impact of Cell-to-Cell Transmission on Viral Spread (PDF)
type: Document
has file: ThesisKumberger_cor_full.pdf
has part: ThesisKumberger_cor_full.pdf

Peter Johannes Manuel Eduard Kumberger
type: Person
given name: Peter Johannes Manuel Eduard
family name: Kumberger

Quantifying the Impact of Cell-to-Cell Transmission on Viral Spread (Other)
type: Document
is version of: Quantifying the Impact of Cell-to-Cell Transmission on Viral Spread (PDF)
is volatile version of: Quantifying the Impact of Cell-to-Cell Transmission on Viral Spread (PDF)
is index codes version of: Quantifying the Impact of Cell-to-Cell Transmission on Viral Spread (PDF)
has file: indexcodes.txt
has part: indexcodes.txt

HTML Summary of #25580 Quantifying the Impact of Cell-to-Cell Transmission on Viral Spread
format: text/html
primary topic: Quantifying the Impact of Cell-to-Cell Transmission on Viral Spread

500 Naturwissenschaften und Mathematik
type: Concept
preferred label: 500 Natural sciences and mathematics @en

570 Biowissenschaften, Biologie
type: Concept
preferred label: 570 Life sciences @en