title: Motility of human renal cells is disturbed by infection with pathogenic hantaviruses
creator: Hägele, Stefan
creator: Müller, Alexander
creator: Nusshag, Christian
creator: Reiser, Jochen
creator: Zeier, Martin
creator: Krautkrämer, Ellen
subject: ddc-610
subject: 610 Medical sciences Medicine
description: Background: Hemorrhagic fever with renal syndrome (HFRS) caused by pathogenic hantaviruses in Europe and Asia is often characterized by acute kidney injury (AKI) with massive proteinuria. Renal filtration depends on the integrity of epithelial and endothelial monolayers in the tubular and glomerular apparatus. Tubular and glomerular cells represent target cells of hantavirus infection. However, the detailed mechanisms of renal impairment induced by hantaviruses are not well understood.    Methods: We analyzed the cellular consequences of hantavirus infection by measuring adhesion and migration capacity of human renal cells infected with Puumala (PUUV) or Hantaan (HTNV) virus. The impact of hantaviral nucleocapsid proteins (N proteins) on motility was examined by transfection of podocytes.    Results: Infection of kidney cells with hantavirus species PUUV and HTNV causes a significant reduction of migration capacity. The impaired motility depends on viral replication and transfection of podocytes with N protein of PUUV or HTNV reveals that the expression of N protein alone is sufficient to deteriorate podocyte function. The cellular effects are more pronounced for the more pathogenic HTNV than for PUUV that causes a milder form of HFRS.    Conclusions: The direct impairment of migration capacity of renal cells by hantaviral N proteins may contribute substantially to proteinuria observed in the clinical picture of hantavirus infection.
publisher: BioMed Central ; Springer
date: 2018
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/25743/1/12879_2018_Article_3583.pdf
identifier: DOI:
identifier: urn:nbn:de:bsz:16-heidok-257436
identifier:   Hägele, Stefan ; Müller, Alexander ; Nusshag, Christian ; Reiser, Jochen ; Zeier, Martin ; Krautkrämer, Ellen  (2018) Motility of human renal cells is disturbed by infection with pathogenic hantaviruses.  BMC Infectious Diseases, 18 (645).  pp. 1-11.  ISSN 1471-2334     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/25743/
rights: info:eu-repo/semantics/openAccess
rights: Please see front page of the work (Sorry, Dublin Core plugin does not recognise license id)
language: eng