eprintid: 25743
rev_number: 18
eprint_status: archive
userid: 1589
dir: disk0/00/02/57/43
datestamp: 2019-01-03 13:58:01
lastmod: 2024-05-06 15:33:19
status_changed: 2019-01-03 13:58:01
type: article
metadata_visibility: show
creators_name: Hägele, Stefan
creators_name: Müller, Alexander
creators_name: Nusshag, Christian
creators_name: Reiser, Jochen
creators_name: Zeier, Martin
creators_name: Krautkrämer, Ellen
title: Motility of human renal cells is disturbed by infection with pathogenic hantaviruses
subjects: ddc-610
divisions: i-910100
keywords: Hantavirus, Kidney, Tubular epithelium, Glomerulus, Podocytes, Proteinuria
cterms_swd: Hanta-Virus
abstract: Background: Hemorrhagic fever with renal syndrome (HFRS) caused by pathogenic hantaviruses in Europe and Asia is often characterized by acute kidney injury (AKI) with massive proteinuria. Renal filtration depends on the integrity of epithelial and endothelial monolayers in the tubular and glomerular apparatus. Tubular and glomerular cells represent target cells of hantavirus infection. However, the detailed mechanisms of renal impairment induced by hantaviruses are not well understood.

Methods: We analyzed the cellular consequences of hantavirus infection by measuring adhesion and migration capacity of human renal cells infected with Puumala (PUUV) or Hantaan (HTNV) virus. The impact of hantaviral nucleocapsid proteins (N proteins) on motility was examined by transfection of podocytes.

Results: Infection of kidney cells with hantavirus species PUUV and HTNV causes a significant reduction of migration capacity. The impaired motility depends on viral replication and transfection of podocytes with N protein of PUUV or HTNV reveals that the expression of N protein alone is sufficient to deteriorate podocyte function. The cellular effects are more pronounced for the more pathogenic HTNV than for PUUV that causes a milder form of HFRS.

Conclusions: The direct impairment of migration capacity of renal cells by hantaviral N proteins may contribute substantially to proteinuria observed in the clinical picture of hantavirus infection.
date: 2018
publisher: BioMed Central ; Springer
id_scheme: DOI
ppn_swb: 1653296267
own_urn: urn:nbn:de:bsz:16-heidok-257436
language: eng
bibsort: HAGELESTEFMOTILITYOF2018
full_text_status: public
publication: BMC Infectious Diseases
volume: 18
number: 645
place_of_pub: London ; Berlin ; Heidelberg
pagerange: 1-11
issn: 1471-2334
citation:   Hägele, Stefan ; Müller, Alexander ; Nusshag, Christian ; Reiser, Jochen ; Zeier, Martin ; Krautkrämer, Ellen  (2018) Motility of human renal cells is disturbed by infection with pathogenic hantaviruses.  BMC Infectious Diseases, 18 (645).  pp. 1-11.  ISSN 1471-2334     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/25743/1/12879_2018_Article_3583.pdf