title: Effects of ranibizumab (Lucentis®) and bevacizumab (Avastin®) on human corneal endothelial cells
creator: Merz, Patrick R.
creator: Röckel, Nina
creator: Ballikaya, Seda
creator: Auffarth, Gerd U.
creator: Schmack, Ingo
subject: ddc-610
subject: 610 Medical sciences Medicine
description: Background: Ingrowth of newly formed blood and lymph vessels (angiogenesis) from the limbus region into the cornea can be treated successfully by subconjunctival application of antiangiogenic agents. Currently, there are several angiogenesis inhibitors from various manufacturers available, such as vascular endothelial growth factor (VEGF) antibodies. The aim of the study was to investigate potential cytotoxic effects of two anti-VEGF agents, ranibizumab (Lucentis®) and bevacizumab (Avastin®) on the human corneal endothelium.    Methods: Human donor corneas, not suitable for corneal transplantation, were organ-cultured in the presence of either ranibizumab (Lucentis®) or bevacizumab (Avastin®) at different concentrations (group 1: 250 μg / ml, group 2: 25 μg / ml, group 3: 2.5 μg / ml) for a period of up to 4 weeks. Microscopic imaging for endothelial cell counting, detection of morphologic alterations of the endothelium, and molecular biology testing (Enzyme-linked Immunosorbent Assay [ELISA]) for metabolic changes was performed.       Results: Background-corrected results showed neither a significant lactate dehydrogenase (LDH) change with increasing culturing time nor a significant difference between ranibizumab (Lucentis®) and bevacizumab (Avastin®) treatment. The endothelial cell density revealed also no statistically significant difference between the two treatment groups with ranibizumab (Lucentis®) and bevacizumab (Avastin®) at all concentrations tested in this study.    Conclusions: In this study, the anti-angiogenic agents ranibizumab (Lucentis®) and bevacizumab (Avastin®) demonstrated no cytotoxic effects on the corneal endothelium of human organ-cultured donor corneas over the limited study time period of 4 weeks. However, based on the study design (in-vitro) and the limited follow-up period, no conclusions on potential long-term effects can be drawn.
publisher: BioMed Central
date: 2018
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/25744/1/12886_2018_Article_978.pdf
identifier: DOI:
identifier: urn:nbn:de:bsz:16-heidok-257445
identifier:   Merz, Patrick R. ; Röckel, Nina ; Ballikaya, Seda ; Auffarth, Gerd U. ; Schmack, Ingo  (2018) Effects of ranibizumab (Lucentis®) and bevacizumab (Avastin®) on human corneal endothelial cells.  BMC Ophthalmology, 18 (316).  pp. 1-8.  ISSN 1471-2415     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/25744/
rights: info:eu-repo/semantics/openAccess
rights: Please see front page of the work (Sorry, Dublin Core plugin does not recognise license id)
language: eng