TY  - GEN
TI  - ADP dependent glucokinase as a novel onco-target for haematological malignancies.
Y1  - 2018///
AV  - public
ID  - heidok25779
A1  - Tandon, Amol
CY  - Heidelberg, Germany
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/25779/
N2  - In this study we aimed at studying the tumor aggressiveness and differentiation potential of Ramos Burkitt?s lymphoma cells upon mitogenic stimulation in light of a novel regulator of immune cell metabolism and activation, ADPGK. We have identified the role of ADPGK in regulation of aerobic glycolysis in Burkitt?s Lymphoma cells and shown that its knock-out leads to reduced tumor aggressiveness, as measured in-vitro via co-culture, migration experiments and metabolic profile, and in-vivo Zebrafish. We found significantly reduced MYC transcription in ADPGK knock-out Burkitt?s lymphoma cells and importantly, several folds reduction in accumulated random mutations in translocated MYC in these cells. We additionally observed a stalled pathway to differentiation of ADPGK knock out B-cells into plasma cells upon stimulation by mitogenic signals. Overall, the study provided the first insights into the role of a novel ER resident protein acting as a regulator of two complementary phenomenon, cell-differentiation and cancer aggressiveness, and thereby opens up new possibilities of therapeutic interventions for hematopoietic malignancies.
ER  -