<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "SNP-ChIP: a versatile and tag-free method to quantify changes in protein binding across the genome"^^ . "Background: Chromatin-immunoprecipitation followed by sequencing (ChIP-seq) is the method of choice for mapping genome-wide binding of chromatin-associated factors. However, broadly applicable methods for between-sample comparisons are lacking. \r\n\r\nResults: Here, we introduce SNP-ChIP, a method that leverages small-scale intra-species polymorphisms, mainly SNPs, for quantitative spike-in normalization of ChIP-seq results. Sourcing spike-in material from the same species ensures antibody cross-reactivity and physiological coherence, thereby eliminating two central limitations of traditional spike-in approaches. We show that SNP-ChIP is robust to changes in sequencing depth and spike-in proportions, and reliably identifies changes in overall protein levels, irrespective of changes in binding distribution. Application of SNP-ChIP to test cases from budding yeast meiosis allowed discovery of novel regulators of the chromosomal protein Red1 and quantitative analysis of the DNA-damage associated histone modification γ-H2AX. \r\n\r\nConclusion: SNP-ChIP is fully compatible with the intra-species diversity of humans and most model organisms and thus offers a general method for normalizing ChIP-seq results."^^ . "2019" . . "20" . "54" . . "BioMed Central ; Springer"^^ . . . "BMC Genomics"^^ . . . "14712164" . . . . . . . . . . . "Tovah E."^^ . "Markowitz"^^ . "Tovah E. Markowitz"^^ . . "Andreas"^^ . "Hochwagen"^^ . "Andreas Hochwagen"^^ . . "Luis A."^^ . "Vale-Silva"^^ . "Luis A. Vale-Silva"^^ . . . . . . "SNP-ChIP: a versatile and tag-free method to quantify changes in protein binding across the genome (PDF)"^^ . . . "12864_2018_Article_5368.pdf"^^ . . . "SNP-ChIP: a versatile and tag-free method to quantify changes in protein binding across the genome (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "SNP-ChIP: a versatile and tag-free method to quantify changes in protein binding across the genome (Other)"^^ . . . . . . "preview.jpg"^^ . . . "SNP-ChIP: a versatile and tag-free method to quantify changes in protein binding across the genome (Other)"^^ . . . . . . "medium.jpg"^^ . . . "SNP-ChIP: a versatile and tag-free method to quantify changes in protein binding across the genome (Other)"^^ . . . . . . "small.jpg"^^ . . . "SNP-ChIP: a versatile and tag-free method to quantify changes in protein binding across the genome (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #25916 \n\nSNP-ChIP: a versatile and tag-free method to quantify changes in protein binding across the genome\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . . . "610 Medizin"@de . "610 Medical sciences Medicine"@en . .