eprintid: 25922
rev_number: 19
eprint_status: archive
userid: 1589
dir: disk0/00/02/59/22
datestamp: 2019-03-06 14:01:44
lastmod: 2024-05-09 01:31:53
status_changed: 2019-03-06 14:01:44
type: article
metadata_visibility: show
creators_name: Yudhistiara, Brian
creators_name: Zwicker, Felix
creators_name: Weber, K. J.
creators_name: Huber, Peter E.
creators_name: Ruehle, Annika
creators_name: Brons, S.
creators_name: Haering, P.
creators_name: Debus, Jürgen
creators_name: Hauswald, S. Henrik
title: The influence of a magnetic field on photon beam radiotherapy in a normal human TK6 lymphoblastoid cell line
subjects: ddc-610
divisions: i-850300
divisions: i-911400
divisions: i-950400
keywords: MRI guided radiotherapy, MR Linac, In-vitro experiment, Normal human cells, TK6 human lymphoblastoid cells
abstract: Background: The implementation of magnetic resonance imaging (MRI) guided radiotherapy (RT) continues to increase. Very limited in-vitro data on the interaction of ionizing radiation and magnetic fields (MF) have been published. In these experiments we focused on the radiation response in a MF of the TK6 human lymphoblastoid cells which are known to be highly radiosensitive due to efficient radiation-induced apoptosis.

Methods: Clonogenicity was determined 12–14 days after irradiation with 1–4 Gy 6 MV photons with or without a 1.0 Tesla MF. Furthermore, alterations in cell cycle distribution and rates of radiation induced apoptosis (FACS analysis of cells with sub-G1 DNA content) were analyzed.

Results: Clonogenic survival showed an exponential dose-dependence, and the radiation sensitivity parameter (α = 1.57/Gy) was in accordance with earlier reports. Upon comparing the clonogenic survival between the two groups, identical results within error bars were obtained. The survival fractions at 2 Gy were 9% (without MF) and 8.5% (with MF), respectively.

Conclusion: A 1.0 Tesla MF does not affect the clonogenicity of TK6 cells irradiated with 1–4 Gy 6MV photons. This supports the use of MRI guided RT, however ongoing research on the interaction of MF and radiotherapy is warranted.
date: 2019
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1663740410
own_urn: urn:nbn:de:bsz:16-heidok-259220
language: eng
bibsort: YUDHISTIARTHEINFLUEN2019
full_text_status: public
publication: Radiation Oncology
volume: 14
number: 11
place_of_pub: London
pagerange: 1-8
issn: 1748-717X
citation:   Yudhistiara, Brian ; Zwicker, Felix ; Weber, K. J. ; Huber, Peter E. ; Ruehle, Annika ; Brons, S. ; Haering, P. ; Debus, Jürgen ; Hauswald, S. Henrik  (2019) The influence of a magnetic field on photon beam radiotherapy in a normal human TK6 lymphoblastoid cell line.  Radiation Oncology, 14 (11).  pp. 1-8.  ISSN 1748-717X     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/25922/1/13014_2019_Article_1212.pdf