<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Automated Correlative Light and\r\nElectron Microscopy using FIB-SEM as\r\na tool to screen for ultrastructural\r\nphenotypes"^^ . "In Correlative Light and Electron Microscopy (CLEM), two imaging modalities are combined\r\nto take advantage of the localization capabilities of light microscopy (LM) to guide the capture\r\nof high-resolution details in the electron microscope (EM). However, traditional approaches\r\nhave proven to be very laborious, thus yielding a too low throughput for quantitative or\r\nexploratory studies of populations. Recently, in the electron microscopy field, FIB-SEM\r\n(Focused Ion Beam -Scanning Electron Microscope) tomography has emerged as a flexible\r\nmethod that enables semi-automated 3D volume acquisitions. During my thesis, I developed\r\nCLEMSite, a tool that takes advantage of the semi-automation and scanning capabilities\r\nof the FIB-SEM to automatically acquire volumes of adherent cultured cells.\r\nCLEMSite is a combination of computer vision and machine learning applications with a library for\r\ncontrolling the microscope ( product from a collaboration with Carl Zeiss GmbH and Fibics Inc.). Thanks to this, the microscope was able to automatically track, find and acquire cell regions previously identified in the light microscope. More specifically, two main modules\r\nwere implemented. First, a correlation module was designed to detect and record reference\r\npoints from a grid pattern present on the culture substrate in both modalities (LM and EM).\r\nSecond, I designed a module that retrieves the regions of interest in the FIB-SEM and that\r\ndrives the acquisition of image stacks between different targets in an unattended fashion. The\r\nautomated CLEM approach is demonstrated on a project where 3D EM volumes are examined\r\nupon multiple siRNA treatments for knocking down genes involved in the morphogenesis\r\nof the Golgi apparatus. Additionally, the power of CLEM approaches using FIB-SEM is\r\ndemonstrated with the detailed structural analysis of two events: the breakage of the nuclear\r\nenvelope within constricted cells and an intriguing catastrophic DNA Damage Response in\r\nbinucleated cells. Our results demonstrate that executing high throughput volume acquisition\r\nin electron microscopy is possible and that EM can provide incredible insights to guide new\r\nbiological discoveries."^^ . "2020" . . . . . . . "Jose Miguel"^^ . "Serra Lleti"^^ . "Jose Miguel Serra Lleti"^^ . . . . . . "Automated Correlative Light and\r\nElectron Microscopy using FIB-SEM as\r\na tool to screen for ultrastructural\r\nphenotypes (PDF)"^^ . . . "Thesis_JoseMiguelSerraLleti.pdf"^^ . . . "Automated Correlative Light and\r\nElectron Microscopy using FIB-SEM as\r\na tool to screen for ultrastructural\r\nphenotypes (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Automated Correlative Light and\r\nElectron Microscopy using FIB-SEM as\r\na tool to screen for ultrastructural\r\nphenotypes (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Automated Correlative Light and\r\nElectron Microscopy using FIB-SEM as\r\na tool to screen for ultrastructural\r\nphenotypes (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Automated Correlative Light and\r\nElectron Microscopy using FIB-SEM as\r\na tool to screen for ultrastructural\r\nphenotypes (Other)"^^ . . . . . . "small.jpg"^^ . . . "Automated Correlative Light and\r\nElectron Microscopy using FIB-SEM as\r\na tool to screen for ultrastructural\r\nphenotypes (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #26154 \n\nAutomated Correlative Light and \nElectron Microscopy using FIB-SEM as \na tool to screen for ultrastructural \nphenotypes\n\n" . "text/html" . . . "004 Informatik"@de . "004 Data processing Computer science"@en . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .