eprintid: 26340
rev_number: 13
eprint_status: archive
userid: 1589
dir: disk0/00/02/63/40
datestamp: 2019-06-17 10:29:05
lastmod: 2019-07-11 11:41:55
status_changed: 2019-06-17 10:29:05
type: article
metadata_visibility: show
creators_name: Schäfer, Richard
creators_name: DeBaun, Malcolm R.
creators_name: Fleck, Erika
creators_name: Centeno, Christopher J.
creators_name: Kraft, Daniela
creators_name: Leibacher, Johannes
creators_name: Bieback, Karen
creators_name: Seifried, Erhard
creators_name: Dragoo, Jason L.
title: Quantitation of progenitor cell populations and growth factors after bone marrow aspirate concentration
subjects: ddc-610
divisions: i-63500
keywords: Bone marrow aspirate concentrate, Bone marrow aspiration, BMAC, Growth factors, Mesenchymal stem cell, Stem cell, Stromal cell
abstract: Background: The number of Mesenchymal Stem/Stromal Cells (MSCs) in the human bone marrow (BM) is small compared to other cell types. BM aspirate concentration (BMAC) may be used to increase numbers of MSCs, but the composition of MSC subpopulations and growth factors after processing are unknown. The purpose of this study was to assess the enrichment of stem/progenitor cells and growth factors in BM aspirate by two different commercial concentration devices versus standard BM aspiration.

Methods: 120 mL of BM was aspirated from the iliac crest of 10 male donors. Each sample was processed simultaneously by either Emcyte GenesisCS® (Emcyte) or Harvest SmartPReP2 BMAC (Harvest) devices and compared to untreated BM aspirate. Samples were analyzed with multicolor flow cytometry for cellular viability and expression of stem/progenitor cells markers. Stem/progenitor cell content was verified by quantification of colony forming unit-fibroblasts (CFU-F). Platelet, red blood cell and total nucleated cell (TNC) content were determined using an automated hematology analyzer. Growth factors contents were analyzed with protein quantification assays. Statistical analyses were performed by ANOVA analysis of variance followed by Tukey’s multiple comparison test or Wilcoxon matched-pairs signed rank test with p < 0.05 for significance.

Results: Cell viability after processing was approximately 90% in all groups. Compared to control, both devices significantly enriched TNCs and platelets, as well as the CD45−CD73+ and CD45−CD73+CD90+ cell populations. Further, Harvest significantly concentrated CD45−CD10+, CD45−CD29+, CD45−CD90+, CD45−CD105+, CD45−CD119+ cells, and CD45dimCD90+CD271+ MSCs, whereas Emcyte significantly enriched CD45dimCD44+CD271+ MSCs. BM concentration also increased the numbers of CFU-F, platelet-derived growth factor, vascular endothelial growth factor, macrophage colony-stimulating factor, interleukin-1b, VCAM-1 and total protein. Neither system concentrated red blood cells, hematopoietic stem cells or bone morphogenetic proteins.

Conclusion: This data could contribute to the development of BMAC quality control assays as both BMAC systems concentrated platelets, growth factors and non-hematopoietic stem cell subpopulations with distinct phenotypes without loss of cell viability when compared to unprocessed BM.
date: 2019
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1667615912
own_urn: urn:nbn:de:bsz:16-heidok-263401
language: eng
bibsort: SCHAFERRICQUANTITATI2019
full_text_status: public
publication: Journal of translational medicine
volume: 17
number: 115
place_of_pub: London
pagerange: 1-9
issn: 1479-5876
citation:   Schäfer, Richard ; DeBaun, Malcolm R. ; Fleck, Erika ; Centeno, Christopher J. ; Kraft, Daniela ; Leibacher, Johannes ; Bieback, Karen ; Seifried, Erhard ; Dragoo, Jason L.  (2019) Quantitation of progenitor cell populations and growth factors after bone marrow aspirate concentration.  Journal of translational medicine, 17 (115).  pp. 1-9.  ISSN 1479-5876     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/26340/1/12967_2019_Article_1866.pdf