%0 Generic
%A Fröhlich, Benjamin
%D 2019
%F heidok:26533
%R 10.11588/heidok.00026533
%T Haemoglobinopathies and the Protection Against  Severe Malaria: Probing Cytoadhesion and Mechanics  of Plasmodium falciparum Infected Erythrocytes
%U https://archiv.ub.uni-heidelberg.de/volltextserver/26533/
%X Haemoglobinopathies and the Protection Against Severe Malaria: Probing Cytoadhesion and  Mechanics of Plasmodium falciparum Infected Erythrocytes  Aim of this thesis was the probing of the cytoadhesion and the mechanical properties of  Plasmodium falciparum infected human wildtype and haemoglobinopathic erythrocytes, to shed  light on the protection mechanism against severe malaria associated with haemoglobin  polymorphisms.  In Chapter 3, the cytoadhesion of infected wildtype and haemoglobinopathic erythrocytes was  probed using solid-supported lipid bilayers functionalized with CD36 or ICAM-1, acting as in vitro  endothelial cell membrane models. Under static conditions, the binding strength was quantified  in terms of the critical pressure of detachment based on cell detachment by laser induced pressure  waves. Additionally, the number of binding events and the critical shear stress of detachment  were determined based on classical parallel plate flow experiments at physiological relevant  shear stresses. Furthermore, the shear stress dependent changes in tight adhesion area were  investigated based on label-free reflection interference contrast microscopy.  In Chapter 4, the parasite induced changes to the erythrocyte mechanics were probed at different  parasite stages using label and contact-free flicker spectroscopy, yielding the membrane bending  modulus, the membrane tension, the harmonic membrane confinement by the cytoskeleton and  the apparent cytosolic viscosity.  Moreover, a comprehensive image of the intraerythrocytic parasite metabolism was generated  using quantitative scanning X-ray fluorescence microscopy.