eprintid: 26706
rev_number: 13
eprint_status: archive
userid: 1589
dir: disk0/00/02/67/06
datestamp: 2019-08-09 10:48:40
lastmod: 2019-09-04 09:16:01
status_changed: 2019-08-09 10:48:40
type: article
metadata_visibility: show
creators_name: Rauber, Conrad
creators_name: Bartelheimer, Katja
creators_name: Zhou, Taotao
creators_name: Rupp, Christian
creators_name: Schnitzler, Paul
creators_name: Schemmer, Peter
creators_name: Sauer, Peter
creators_name: Weiss, Karl Heinz
creators_name: Gotthardt, Daniel Nils
title: Prevalence of human herpesviruses in biliary fluid and their association with biliary complications after liver transplantation
subjects: ddc-610
divisions: i-910100
divisions: i-910200
keywords: Herpesvirus, HHV-6, Bile, Stricture, Non-anastomotic stricture, Liver transplantation, Complication
abstract: Background: Beta-herpesviruses are common opportunistic pathogens that cause morbidity after liver transplantation (LT).

Methods: Objective of the study was to evaluate the prevalence and correlation of herpesviruses in bile, blood and liver tissue and to investigate their association with biliary complications and retransplantation (re-LT) free survival after LT. The study design is a single-center case-control study. We performed quantative polymerase chain reaction (qPCR) for herpesvirus 1–8 DNA in bile, blood and liver tissue of 73 patients after first LT and analyzed their clinical courses retrospectively.

Results: The median follow-up was 48 months (range 2–102), during which a total of 16 patients underwent re-LT and 11 patients died. Of the patients, 46.5% received valganciclovir prophylaxis at the time of bile sample acquisition. Cytomegalovirus (CMV) (18.3%), human herpesvirus 6 (HHV-6) (34.2%), human herpesvirus 7 (HHV-7) (20.5%) and Epstein-Barr virus (EBV) (16.4%) were highly prevalent in bile after LT, while herpes simpex virus 1 and 2 (HSV-1, HSV-2), varicella-zoster virus (VZV) and human herpesvirus 8 (HHV-8) were not or rarely detected in bile. Valganciclovir prophylaxis did not reduce the prevalence of HHV-6 and HHV-7 in bile, but it did reduce the presence of CMV and EBV. The presence of HHV-6 in bile was associated with non-anastomotic biliary strictures (NAS) and acute cellular rejection (ACR).

Conclusions: CMV, EBV, HHV-6 and HHV-7 are more prevalent in biliary fluid than in liver biopsy or blood serum after LT. HHV-6 and HHV-7 might be associated with biliary complications after LT. Biliary fluids might be an attractive target for routine herpesvirus detection.
date: 2019
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1675884897
own_urn: urn:nbn:de:bsz:16-heidok-267062
language: eng
bibsort: RAUBERCONRPREVALENCE2019
full_text_status: public
publication: BMC Gastroenterology
volume: 19
number: 110
place_of_pub: London
pagerange: 1-10
issn: 1471-230X
citation:   Rauber, Conrad ; Bartelheimer, Katja ; Zhou, Taotao ; Rupp, Christian ; Schnitzler, Paul ; Schemmer, Peter ; Sauer, Peter ; Weiss, Karl Heinz ; Gotthardt, Daniel Nils  (2019) Prevalence of human herpesviruses in biliary fluid and their association with biliary complications after liver transplantation.  BMC Gastroenterology, 19 (110).  pp. 1-10.  ISSN 1471-230X     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/26706/1/12876_2019_Article_1033.pdf