TY  - JOUR
TI  - Leaf extract of Caesalpinia mimosoides enhances oxidative stress resistance and prolongs lifespan in Caenorhabditis elegans
SP  - 1
JF  - BMC Complementary and Alternative Medicine
ID  - heidok26799
AV  - public
VL  - 19
EP  - 13
PB  - BioMed Central
Y1  - 2019///
IS  - 164
KW  - Caesalpinia mimosoides
KW  -  Caenorhabditis elegans
KW  -  Antioxidant
KW  -  Oxidative stress
KW  -  Aging
KW  -  DAF-16
SN  - 1472-6882
A1  - Rangsinth, Panthakarn
A1  - Prasansuklab, Anchalee
A1  - Duangjan, Chatrawee
A1  - Gu, Xiaojie
A1  - Meemon, Krai
A1  - Wink, Michael
A1  - Tencomnao, Tewin
CY  - London
N2  - Background: Caesalpinia mimosoides, a vegetable consumed in Thailand, has been reported to exhibit in vitro antioxidant properties. The in vivo antioxidant and anti-aging activities have not been investigated. The aim of this research was to study the antioxidant activity of C. mimosoides extracts in Caenorhabditis elegans, a widely used model organism in this context.

Methods: C. elegans were treated with C. mimosoides extracts in a various concentrations. To investigate the protective effects of the extract against oxidative stress, wild-type N2 were used to determine survival rate under oxidative stress and intracellular ROS. To study underlying mechanisms, the mutant strains with GFP reporter gene including TJ356, CF1553, EU1 and LD4 were used to study DAF-16, SOD-3, SKN-1 and GST-4 gene, respectively. Lifespan and aging pigment of the worms were also investigated.

Results: A leaf extract of C. mimosoides improved resistance to oxidative stress and reduced intracellular ROS accumulation in nematodes. The antioxidant effects were mediated through the DAF-16/FOXO pathway and SOD-3 expression, whereas the expression of SKN-1 and GST-4 were not altered. The extract also prolonged lifespan and decreased aging pigments, while the body length and brood size of the worms were not affected by the extract, indicating low toxicity and excluding dietary restriction.

Conclusions: The results of this study establish the antioxidant activity of C. mimosoides extract in vivo and suggest its potential as a dietary supplement and alternative medicine to defend against oxidative stress and aging, which should be investigated in intervention studies.
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/26799/
ER  -