eprintid: 26799
rev_number: 16
eprint_status: archive
userid: 1589
dir: disk0/00/02/67/99
datestamp: 2019-08-13 08:51:47
lastmod: 2019-09-22 10:40:55
status_changed: 2019-08-13 08:51:47
type: article
metadata_visibility: show
creators_name: Rangsinth, Panthakarn
creators_name: Prasansuklab, Anchalee
creators_name: Duangjan, Chatrawee
creators_name: Gu, Xiaojie
creators_name: Meemon, Krai
creators_name: Wink, Michael
creators_name: Tencomnao, Tewin
title: Leaf extract of Caesalpinia mimosoides enhances oxidative stress resistance and prolongs lifespan in Caenorhabditis elegans
subjects: 610
divisions: 160100
keywords: Caesalpinia mimosoides, Caenorhabditis elegans, Antioxidant, Oxidative stress, Aging, DAF-16
abstract: Background: Caesalpinia mimosoides, a vegetable consumed in Thailand, has been reported to exhibit in vitro antioxidant properties. The in vivo antioxidant and anti-aging activities have not been investigated. The aim of this research was to study the antioxidant activity of C. mimosoides extracts in Caenorhabditis elegans, a widely used model organism in this context.

Methods: C. elegans were treated with C. mimosoides extracts in a various concentrations. To investigate the protective effects of the extract against oxidative stress, wild-type N2 were used to determine survival rate under oxidative stress and intracellular ROS. To study underlying mechanisms, the mutant strains with GFP reporter gene including TJ356, CF1553, EU1 and LD4 were used to study DAF-16, SOD-3, SKN-1 and GST-4 gene, respectively. Lifespan and aging pigment of the worms were also investigated.

Results: A leaf extract of C. mimosoides improved resistance to oxidative stress and reduced intracellular ROS accumulation in nematodes. The antioxidant effects were mediated through the DAF-16/FOXO pathway and SOD-3 expression, whereas the expression of SKN-1 and GST-4 were not altered. The extract also prolonged lifespan and decreased aging pigments, while the body length and brood size of the worms were not affected by the extract, indicating low toxicity and excluding dietary restriction.

Conclusions: The results of this study establish the antioxidant activity of C. mimosoides extract in vivo and suggest its potential as a dietary supplement and alternative medicine to defend against oxidative stress and aging, which should be investigated in intervention studies.
date: 2019
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1671873327
own_urn: urn:nbn:de:bsz:16-heidok-267994
language: eng
bibsort: RANGSINTHPLEAFEXTRAC2019
full_text_status: public
publication: BMC Complementary and Alternative Medicine
volume: 19
number: 164
place_of_pub: London
pagerange: 1-13
issn: 1472-6882
citation:   Rangsinth, Panthakarn ; Prasansuklab, Anchalee ; Duangjan, Chatrawee ; Gu, Xiaojie ; Meemon, Krai ; Wink, Michael ; Tencomnao, Tewin  (2019) Leaf extract of Caesalpinia mimosoides enhances oxidative stress resistance and prolongs lifespan in Caenorhabditis elegans.  BMC Complementary and Alternative Medicine, 19 (164).  pp. 1-13.  ISSN 1472-6882     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/26799/1/12906_2019_Article_2578.pdf