eprintid: 26827
rev_number: 12
eprint_status: archive
userid: 1589
dir: disk0/00/02/68/27
datestamp: 2019-08-14 10:34:34
lastmod: 2019-09-23 15:43:41
status_changed: 2019-08-14 10:34:34
type: article
metadata_visibility: show
creators_name: Selmoni, Oliver M.
creators_name: Maitre, Diane
creators_name: Roux, Julien
creators_name: Wilkins, Laetitia G. E.
creators_name: Marques da Cunha, Lucas
creators_name: Vermeirssen, Etienne L. M.
creators_name: Knörr, Susanne
creators_name: Robinson-Rechavi, Marc
creators_name: Wedekind, Claus
title: Sex-specific changes in gene expression in response to estrogen pollution around the onset of sex differentiation in grayling (Salmonidae)
subjects: ddc-570
subjects: ddc-610
divisions: i-721000
abstract: The synthetic 17α-ethinylestradiol (EE2) is a common estrogenic pollutant that has been suspected to affect the demography of river-dwelling salmonids. One possibility is that exposure to EE2 tips the balance during initial steps of sex differentiation, so that male genotypes show female-specific gene expression and gonad formation. Here we study EE2 effects on gene expression around the onset of sex differentiation in a population of European grayling (Thymallus thymallus) that suffers from sex ratio distortions. We exposed singly-raised embryos to one dose of 1 ng/L EE2, studied gene expression 10 days before hatching, at the day of hatching, and around the end of the yolk-sac stage, and related it to genetic sex (sdY genotype). We found that exposure to EE2 affects expression of a large number of genes, especially around hatching. These effects were strongly sex-dependent. We then raised fish for several months after hatching and found no evidence of sex reversal in the EE2-exposed fish. We conclude that ecologically relevant (i.e. low) levels of EE2 pollution do not cause sex reversal by simply tipping the balance at early stages of sex differentiation, but that they interfere with sex-specific gene expression.
date: 2019
publisher: BioMed Central ; Springer
id_scheme: DOI
ppn_swb: 1672107881
own_urn: urn:nbn:de:bsz:16-heidok-268274
language: eng
bibsort: SELMONIOLISEXSPECIFI2019
full_text_status: public
publication: BMC Genomics
volume: 20
number: 583
place_of_pub: London ; Berlin, Heidelberg
pagerange: 1-12
issn: 1471-2164
citation:   Selmoni, Oliver M. ; Maitre, Diane ; Roux, Julien ; Wilkins, Laetitia G. E. ; Marques da Cunha, Lucas ; Vermeirssen, Etienne L. M. ; Knörr, Susanne ; Robinson-Rechavi, Marc ; Wedekind, Claus  (2019) Sex-specific changes in gene expression in response to estrogen pollution around the onset of sex differentiation in grayling (Salmonidae).  BMC Genomics, 20 (583).  pp. 1-12.  ISSN 1471-2164     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/26827/1/12864_2019_Article_5955.pdf