eprintid: 26886
rev_number: 13
eprint_status: archive
userid: 4576
dir: disk0/00/02/68/86
datestamp: 2019-08-29 09:47:10
lastmod: 2019-10-01 16:06:20
status_changed: 2019-08-29 09:47:10
type: doctoralThesis
metadata_visibility: show
creators_name: Viehweger, Florian
title: Inhibition/knock down and stimulation of the canonical WNT/ß Catenin signaling pathway in rhabdomyosarcoma
title_de: Inhibition/Knock-down und Stimulation des kanonischen WNT/ß-Catenin-Signalweges im Rhabdomyosarkom
subjects: 610
divisions: 62900
adv_faculty: af-06
cterms_swd: Rhabdomyosarkom
cterms_swd: WNT Signalwegs
cterms_swd: ß-Catenin
abstract: The development of skeletal muscle from immature precursors is partially driven by canonical WNT/β-catenin signaling. Rhabdomyosarcomas (RMS) are immature skeletal muscle-like, highly lethal cancers with a variably pronounced blockade of muscle differentiation. To investigate whether canonical β-catenin signaling in RMS is involved in differentiation and aggressiveness of RMS, we analyzed the effects of WNT3A and of a siRNA-mediated or pharmacologically induced β-catenin knock-down on proliferation, apoptosis and differentiation of embryonal and alveolar RMS cell lines. While the canonical WNT pathway was maintained in all cell lines as shown by WNT3A induced AXIN expression, more distal steps including transcriptional activation of its key target genes were consistently impaired. In addition, activation or inhibition of canonical WNT/β-catenin only moderately affected proliferation, apoptosis or myodifferentiation of the RMS tumor cells. Together our data indicates a subordinary role of the canonical WNT/β-catenin signaling for RMS proliferation, apoptosis or differentiation and thus aggressiveness of this malignant childhood tumor.
date: 2019
id_scheme: PubMed
ppn_swb: 1676168354
own_urn: urn:nbn:de:bsz:16-heidok-268868
date_accepted: 2019-07-03
advisor: HASH(0x564e1c471b90)
language: ger
bibsort: VIEHWEGERFINHIBITION2019
full_text_status: public
citation:   Viehweger, Florian  (2019) Inhibition/knock down and stimulation of the canonical WNT/ß Catenin signaling pathway in rhabdomyosarcoma.  [Dissertation]     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/26886/1/Viehweger_Florian%20Doktorarbeit_17_11_18_3.pdf