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primary topic: Investigation of Cancerous Tissues by MALDI Mass Spectrometry Imaging - Imaging of proteolytic activity in frozen tissue and standardised on-tissue digestion

Investigation of Cancerous Tissues by MALDI Mass Spectrometry Imaging - Imaging of proteolytic activity in frozen tissue and standardised on-tissue digestion
type: Article
abstract: Multiple alterations of a normal cell can lead to a localised tumour that potentially develops the ability to become invasive and metastasize. Proteolysis of the surrounding tissue environment is driven by proteases, as they are involved in degradation of e.g. extracellular matrix. Proteases are a class of tightly regulated enzymes that hydrolyse peptide bonds. To investigate the spatial distribution of enzyme activity, typically fluorescence-based substrate reporters are developed for in vivo and in vitro applica¬tions. The design of such chemical probes is complex and requires knowledge about the target enzymes’ specificity. The topical application of fluorescent probes to frozen tissue has rarely been done but enables the visualization of specific proteolytic activity. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is an analytical tool with increasing acceptance for tissue analysis in pre-clinical applications. The technique offers the spatially resolved analysis of hundreds of molecules at a time by their mass-to-charge ratio (m/z). Enabling the visualization of protease activity by MALDI MSI, without the necessity of extensive probe design, would have a major impact on the investigation of proteases in cancerous tissue. An absolute requirement for all newly developed methods, but especially if they should be transferred to clinical applications, is repeatability and reproducibility. However, MALDI MSI studies currently still lack a rational, score-based evaluation of repeatability. Thus, this thesis had the following aims: 1. Develop a method for monitoring of tissue protease activity by using MALDI MSI on different porcine tissues. 2. Validate the developed method by their time-dependence, concentration-dependence of chemical inhibition, as well as by the identification of specific protein degradation products. Furthermore, use a gastric tumour mouse model to evaluate differences in protease activity for tumorous tissue co…
date: 2019
author list: authors
type: EPrint
type: DoctoralThesisEPrint
is part of: repository
has document: Investigation of Cancerous Tissues by MALDI Mass Spectrometry Imaging - Imaging of proteolytic activity in frozen tissue and standardised on-tissue digestion (PDF)
has document: Investigation of Cancerous Tissues by MALDI Mass Spectrometry Imaging - Imaging of proteolytic activity in frozen tissue and standardised on-tissue digestion (Other)
subject: 600 Technik, Medizin, angewandte Wissenschaften

Investigation of Cancerous Tissues by MALDI Mass Spectrometry Imaging - Imaging of proteolytic activity in frozen tissue and standardised on-tissue digestion (PDF)
type: Document
has file: Dissertation Katrin Erich.pdf
has part: Dissertation Katrin Erich.pdf

Katrin Erich
type: Person
given name: Katrin
family name: Erich

Investigation of Cancerous Tissues by MALDI Mass Spectrometry Imaging - Imaging of proteolytic activity in frozen tissue and standardised on-tissue digestion (Other)
type: Document
is version of: Investigation of Cancerous Tissues by MALDI Mass Spectrometry Imaging - Imaging of proteolytic activity in frozen tissue and standardised on-tissue digestion (PDF)
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primary topic: Investigation of Cancerous Tissues by MALDI Mass Spectrometry Imaging - Imaging of proteolytic activity in frozen tissue and standardised on-tissue digestion

600 Technik, Medizin, angewandte Wissenschaften
type: Concept
preferred label: 600 Technology (Applied sciences) @en