eprintid: 26963
rev_number: 12
eprint_status: archive
userid: 1589
dir: disk0/00/02/69/63
datestamp: 2019-08-21 10:24:54
lastmod: 2019-10-01 13:40:21
status_changed: 2019-08-21 10:24:54
type: article
metadata_visibility: show
creators_name: Eisenmann, Birgit
creators_name: Czemmel, Stefan
creators_name: Ziegler, Tobias
creators_name: Buchholz, Günther
creators_name: Kortekamp, Andreas
creators_name: Trapp, Oliver
creators_name: Rausch, Thomas
creators_name: Dry, Ian
creators_name: Bogs, Jochen
title: Rpv3–1 mediated resistance to grapevine downy mildew is associated with specific host transcriptional responses and the accumulation of stilbenes
subjects: 610
divisions: 721000
keywords: Disease resistance, Downy mildew, Grapevine, Plasmopara viticola, Stilbenes, Vitis vinifera, Metabolomics, Rpv3
abstract: Background: European grapevine cultivars (Vitis vinifera spp.) are highly susceptible to the downy mildew pathogen Plasmopara viticola. Breeding of resistant V. vinifera cultivars is a promising strategy to reduce the impact of disease management. Most cultivars that have been bred for resistance to downy mildew, rely on resistance mediated by the Rpv3 (Resistance to P. viticola) locus. However, despite the extensive use of this locus, little is known about the mechanism of Rpv3-mediated resistance.

Results: In this study, Rpv3-mediated defense responses were investigated in Rpv3+ and Rpv3ˉ grapevine cultivars following inoculation with two distinct P. viticola isolates avrRpv3+ and avrRpv3ˉ, with the latter being able to overcome Rpv3 resistance. Based on comparative microscopic, metabolomic and transcriptomic analyses, our results show that the Rpv3–1-mediated resistance is associated with a defense mechanism that triggers synthesis of fungi-toxic stilbenes and programmed cell death (PCD), resulting in reduced but not suppressed pathogen growth and development. Functional annotation of the encoded protein sequence of genes significantly upregulated during the Rpv3–1-mediated defense response revealed putative roles in pathogen recognition, signal transduction and defense responses.

Conclusion: This study used histochemical, transcriptomic and metabolomic analyses of Rpv3+ and susceptible cultivars inoculated with avirulent and virulent P. viticola isolates to investigate mechanism underlying the Rpv3–1-mediated resistance response. We demonstrated a strong correlation between the expressions of stilbene biosynthesis related genes, the accumulation of fungi-toxic stilbenes, pathogen growth inhibition and PCD.
date: 2019
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 167792733X
own_urn: urn:nbn:de:bsz:16-heidok-269639
language: eng
bibsort: EISENMANNBRPV31MEDIA2019
full_text_status: public
publication: BMC Plant Biology
volume: 19
number: 343
place_of_pub: London
pagerange: 1-17
issn: 1471-2229
citation:   Eisenmann, Birgit ; Czemmel, Stefan ; Ziegler, Tobias ; Buchholz, Günther ; Kortekamp, Andreas ; Trapp, Oliver ; Rausch, Thomas ; Dry, Ian ; Bogs, Jochen  (2019) Rpv3–1 mediated resistance to grapevine downy mildew is associated with specific host transcriptional responses and the accumulation of stilbenes.  BMC Plant Biology, 19 (343).  pp. 1-17.  ISSN 1471-2229     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/26963/1/12870_2019_Article_1935.pdf