<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Molecular Screening for Vel- Blood Donors in Southwestern Germany"^^ . "BACKGROUND: The SMIM1 protein carries the Vel blood group antigen, and homozygosity for a 17 bp deletion in the coding region of the SMIM1 gene represents the molecular basis of the Vel- blood group phenotype. We developed PCR-based methods for typing the SMIM1 17 bp (64-80del) gene deletion and performed a molecular screening for the Vel- blood type in German blood donors. \r\n\r\nMETHODS: For SMIM1 genotyping, TaqMan-PCR and PCRSSP methods were developed and validated using reference samples. Both methods were used for screening of donors with blood group O from southwestern Germany. Heterozygotes and homozygotes for the SMIM1 64-80del allele were serologically typed for the Vel blood group antigen. In addition, the rs1175550 SNP in SMIM1 was typed and correlated to the results of the phenotyping. \r\n\r\nRESULTS: Both genotyping methods, TaqMan-PCR and PCR-SSP, represent reliable methods for the detection of the SMIM1 64-80del allele. Screening of 10,598 blood group O donors revealed 5 individuals homozygous for the deletional allele. They were confirmed Vel- by serological typing. Heterozygotes for the 64-80del allele showed different antigen expressions ranging from very weak to regular positive. \r\n\r\nCONCLUSION: Molecular screening of blood donors for the Vel- blood type is feasible and avoids the limitations of serological typing which might show false-negative results with heterozygous individuals. The identification of Vel- blood donors significantly contributes to the adequate blood supply of patients with anti-Vel."^^ . "2015" . . . "42" . "6" . . "Karger"^^ . . . "Transfusion Medicine and Hemotherapy"^^ . . . "1660379616603818" . . . . . . . . . . . . . . . . . . . . "Erwin A."^^ . "Scharberg"^^ . "Erwin A. Scharberg"^^ . . "Naime"^^ . "Kömürcü"^^ . "Naime Kömürcü"^^ . . "Sina"^^ . "Rothenberger"^^ . "Sina Rothenberger"^^ . . "Gabi"^^ . "Rink"^^ . "Gabi Rink"^^ . . "Peter"^^ . "Bugert"^^ . "Peter Bugert"^^ . . "Carola"^^ . "Wieckhusen"^^ . "Carola Wieckhusen"^^ . . . . . . "Molecular Screening for Vel- Blood Donors in Southwestern Germany (PDF)"^^ . . . "440791.pdf"^^ . . . "Molecular Screening for Vel- Blood Donors in Southwestern Germany (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #27236 \n\nMolecular Screening for Vel- Blood Donors in Southwestern Germany\n\n" . "text/html" . . . "610 Medizin"@de . "610 Medical sciences Medicine"@en . .