<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "A Single-Tube Real-Time PCR Assay for Mycoplasma Detection as a Routine Quality Control of Cell Therapeutics"^^ . "BACKGROUND: Contamination of cell culture and biological material by mollicute species is an important safety issue and requires testing. We have developed a singletube real-time polymerase chain reaction (PCR) assay for rapid detection of Mollicutes species stipulated by the European Pharmacopeia. \r\n\r\nMETHODS: Primers and TaqMan probes (FAM- abeled) were deduced from 16S rDNA sequence alignment of 18 mollicutes species. A synthetic internal control (IC) DNA and an IC-specific TaqMan probe (VIC-labeled) were included. The analytical sensitivity of the assay was determined on DNA dilutions from 12 mollicute strains. Specificity was proven by the use of DNA from other bacteria. \r\n\r\nRESULTS: Analytical sensitivities of the PCR assay were in the range of 405–2,431 genomes/ml for 11 of the 12 tested mollicute DNA samples. The lowest sensitivity was found for Ureaplasma urealyticum (19,239 genomes/ml). Negative results for DNA samples from 3 different ubiquitous bacteria demonstrated the specificity of the PCR assay for Mollicutes. Direct testing of cell culture supernatants spiked with Mycoplasma orale revealed similar sensitivity compared to isolated DNA. \r\n\r\nCONCLUSION: Our single-tube real-time PCR assay with internal reaction control enables rapid and specific detection of mollicute contaminants. The test protocol is suitable for routine quality control of cell therapeutics."^^ . "2014" . . . "41" . "1" . . "Karger"^^ . . . "Transfusion Medicine and Hemotherapy"^^ . . . "1660379616603818" . . . . . . . . . . . . . . . . . . . . "Peter"^^ . "Bugert"^^ . "Peter Bugert"^^ . . "Gabi"^^ . "Rink"^^ . "Gabi Rink"^^ . . "Karen"^^ . "Bieback"^^ . "Karen Bieback"^^ . . "Andrea"^^ . "Hecker"^^ . "Andrea Hecker"^^ . . "Karin"^^ . "Janetzko"^^ . "Karin Janetzko"^^ . . "Harald"^^ . "Klüter"^^ . "Harald Klüter"^^ . . . . . . "A Single-Tube Real-Time PCR Assay for Mycoplasma Detection as a Routine Quality Control of Cell Therapeutics (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "A Single-Tube Real-Time PCR Assay for Mycoplasma Detection as a Routine Quality Control of Cell Therapeutics (PDF)"^^ . . . "357096.pdf"^^ . . "HTML Summary of #27237 \n\nA Single-Tube Real-Time PCR Assay for Mycoplasma Detection as a Routine Quality Control of Cell Therapeutics\n\n" . "text/html" . . . "610 Medizin"@de . "610 Medical sciences Medicine"@en . .