title: Platelet Lysate as Replacement for Fetal Bovine Serum in Mesenchymal Stromal Cell Cultures
creator: Bieback, Karen
subject: ddc-610
subject: 610 Medical sciences Medicine
description: Mesenchymal stromal cells (MSC) emerged as highly attractive in cell-based regenerative medicine. Initially thought to provide cells capable of differentiation towards mesenchymal cell types (osteoblasts, chondrocytes, adipocytes etc.), by and by potent immunoregulatory and pro-regenerative activities have been discovered, broadening the field of potential applications from bone and cartilage regeneration to wound healing and treatment of autoimmune diseases. Due to the limited frequency in most tissue sources, ex vivo expansion of MSC is required compliant with good manufacturing practice (GMP) guidelines to yield clinically relevant cell doses. Though, still most manufacturing protocols use fetal bovine serum (FBS) as cell culture supplement to isolate and to expand MSC. However, the high lot-to-lot variability as well as risk of contamination and immunization call for xenogenic-free culture conditions. In terms of standardization, chemically defined media appear as  the ultimate achievement. Since these media need to maintain all key cellular and therapy-relevant features of MSC, the development of chemically defined media is still – albeit highly investigated – only in its beginning. The current alternatives to FBS rely on human blood-derived components: plasma, serum, umbilical cord blood serum, and platelet derivatives like platelet lysate. Focusing on quality aspects, the latter will be addressed within this review.
publisher: Karger
date: 2013
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/27239/5/354061.pdf
identifier: DOI:10.11588/heidok.00027239
identifier: https://doi.org/10.1159/000354061
identifier: urn:nbn:de:bsz:16-heidok-272391
identifier:   Bieback, Karen  (2013) Platelet Lysate as Replacement for Fetal Bovine Serum in Mesenchymal Stromal Cell Cultures.  Transfusion Medicine and Hemotherapy, 40 (5).  pp. 326-335.  ISSN 1660-3796 (Druck-Ausg.), 1660-3818 (Online-Ausg.)     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/27239/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng