<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Golgi apparatus and Golgi outposts in neurons\r\nstudied by correlative microscopy"^^ . "The Golgi apparatus is a highly dynamic organelle, which has many vital functions in\r\ncellular mechanisms like lipid metabolism, protein secretion, intracellular signaling, and\r\nregulation of cell division. First described in neurons, the ultrastructure and function of\r\nthis complex network is still not well delineated. Recent studies have shown this\r\nsecretory organelle also in dendrites of neurons, named Golgi outposts (GOs). To date,\r\nthese GOs have been analyzed mainly by light microscopy while their ultrastructural\r\nappearance remained only poorly defined.\r\nTo study the morphology of GOs in dendrites and to examine if GOs show a\r\ndifferent ultrastructure to that of somatic Golgi apparatus, I established new applications\r\nof correlative light and electron microscopy in cultured neurons and brain slices, which\r\ncombine fluorescence microscopy with the superior resolution of electron microscopy.\r\nPhoto-oxidation-based approaches allow for the direct ultra-structural visualization of\r\nfluorescent markers in 3D, like genetically encoded green fluorescent proteins (GFPs).\r\nHowever, this challenging methodology had to be adapted to detect Golgi enzymes in\r\nneuronal cell cultures and brain tissue. Therefore, I first optimized the chemical fixation\r\nconditions for cultured hippocampal neurons to approximate the morphologic quality of\r\nhigh-pressure freeze fixation. Secondly, I enhanced sensitivity, reliability, and precision\r\nof photo-oxidation procedures on neurons, which allowed to analyse the 3D\r\nultrastructure of GOs in dendrites of cultured neurons and mammalian brain slices.\r\nCorrelative 3D microscopy showed that GOs are smaller in volume and have\r\nlower number of cisternae per stack compared to somatic Golgi apparatus of the same\r\ncell. Further, the number of peri-Golgi vesicles around GOs appears to be less.\r\nAdditionally, while the stack polarity of somatic Golgi apparatus might switch along the\r\nribbon, GOs have strictly unidirectional polarity.\r\nThe technical achievements and enhanced sensitivity regarding correlative\r\nmicroscopy of GFPs in cultured neurons and brain slices allow for further ultrastructural\r\ninvestigations of GOs at different metabolic states to better understand their vital\r\nfunctions."^^ . "2020" . . . . . . . "Areej"^^ . "Albariri"^^ . "Areej Albariri"^^ . . . . . . "Golgi apparatus and Golgi outposts in neurons\r\nstudied by correlative microscopy (PDF)"^^ . . . "PhD_Areej Albariri_pdf_.pdf"^^ . . . "Golgi apparatus and Golgi outposts in neurons\r\nstudied by correlative microscopy (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #27330 \n\nGolgi apparatus and Golgi outposts in neurons \nstudied by correlative microscopy\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .