<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Detection of bacteria and virus-associated\r\nPancreatic Ductal Adenocarcinoma by\r\ncell-free protein microarray"^^ . "Summary\r\nBackground\r\nInfection is the cause of nearly 20% of all cancers. Infectious agents such as the bacterium\r\nHelicobacter pylori (H. pylori) as the first known bacterial carcinogen and viruses from\r\nHerpesviridae family are involved in the pathogenesis of different cancers. Screening for\r\nantibodies against bacterial and viral antigens in cancer patients may therefore uncover\r\npotential markers and targets for optimized cancer control strategies. To these ends, I\r\nstudied the antibody composition in the peripheral blood of patients with pancreatic ductal\r\nadenocarcinoma (PDAC) and appropriate non-cancer donors as controls.\r\nMethods\r\nAll 1437 open reading frames (ORFs) of H. pylori and the 100 ORFs of Turkey herpesvirus\r\n(HVT) were produced by two successive PCRs and spotted on nickel-coated glass surfaces. By\r\nin vitro transcription-translation, whole-proteome microarrays were produced for H. pylori\r\nand HVT. Immunoassays were performed with PDAC and non-PDAC serum samples to detect\r\nantibody binding patterns and to differentiate between PDAC and non-PDAC groups. For\r\npresenting the association pattern of each detected antigen with PDAC, the result was\r\ncalculated as Odds Ratio (OR) with 95% Confidence Interval (CI). Also, functional annotations\r\nwere performed.\r\nResults\r\nAt first, the H. pylori proteome was screened using serum pools of 10 PDAC, 10 healthy and\r\n9 CP (chronic pancreatitis). A total of 46 proteins were detected, of which 29 showed\r\nassociation to PDAC (OR>1). After screening for candidates in the serum pools, individual\r\nserum samples of PDAC and non-PDAC (healthy and CP) were applied to the microarray. In\r\ntotal, 47 PDAC and 38 non-PDAC samples (19 healthy and 19 CP) were analyzed. A total of\r\n62 proteins were detected. Most of them showed association to PDAC (OR>1); 33 antigens\r\nhad also been found in the pool experiments, such as HP0874, HP0599 and HP0175. In\r\nfurther experiments, 11 PDAC and 8 non-PDAC serum pools with gastrointestinal conditions\r\nwere applied to find H. pylori positive antigens. In total, 59 antigens were detected in this\r\nexperiment of which 29 showed association to PDAC (OR>1).\r\nThe HVT proteome was firstly screened using serum pools (10 PDAC, 10 healthy and 10 CP).\r\nIn this step, three antigens were detected in PDAC pools. Subsequently, screening was\r\nperformed using individual samples. Altogether, 74 PDAC and 46 non-PDAC serum samples\r\n(24 CP patients and 22 healthy donors) were applied to the HVT microarray. In total, 25 HVT\r\ncandidates were identified including the antigens which were detected in the pools:\r\nHVT079, HVT059 and HVT062.\r\nConclusions\r\nH. pylori and HVT screenings were performed to find antigens which are associated with the\r\noccurrence of PDAC using serum pools and individual serum samples. Each screening\r\nproduced a candidate list, which is a valuable resource for diagnostic purposes. Among the64\r\n167 H. pylori detected antigens, four antigens were detected in all three datasets with strong\r\nassociation to PDAC: HP0874, HP0010, HP0601 and HP01238. Among the 25 HVT proteins\r\nthat were detected, three were detected commonly: HVT079, HVT059 and HVT062. These\r\nresults could help PDAC diagnostics and provides targets for interfering with PDAC\r\ndevelopment and progression."^^ . "2020" . . . . . . . "Soroosh"^^ . "Shahryarhesami"^^ . "Soroosh Shahryarhesami"^^ . . . . . . "Detection of bacteria and virus-associated\r\nPancreatic Ductal Adenocarcinoma by\r\ncell-free protein microarray (PDF)"^^ . . . "PhD Thesis_Soroosh.pdf"^^ . . . "Detection of bacteria and virus-associated\r\nPancreatic Ductal Adenocarcinoma by\r\ncell-free protein microarray (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #28115 \n\nDetection of bacteria and virus-associated \nPancreatic Ductal Adenocarcinoma by \ncell-free protein microarray\n\n" . "text/html" . . . "000 Allgemeines, Wissenschaft, Informatik"@de . "000 Generalities, Science"@en . .