TY - JOUR VL - 2019 EP - 9 ID - heidok28283 CY - London, New York JF - Nature Methods A1 - Berg, Stuart A1 - Kutra, Dominik A1 - Kroeger, Thorben A1 - Straehle, Christoph N. A1 - Kausler, Bernhard X. A1 - Haubold, Carsten A1 - Schiegg, Martin A1 - Ales, Janez A1 - Beier, Thorsten A1 - Rudy, Markus A1 - Eren, Kemal A1 - Cervantes, Jaime I A1 - Xu, Buote A1 - Beuttenmueller, Fynn A1 - Wolny, Adrian A1 - Zhang, Chong A1 - Koethe, Ullrich A1 - Hamprecht, Fred A. A1 - Kreshuk, Anna AV - public IS - 16 PB - Nature Publishing Group SN - 1548-7105 (Online-Ausg.), 1548-7091 (Druck-Ausg.) N2 - We present ilastik, an easy-to-use interactive tool that brings machine-learning-based (bio)image analysis to end users without substantial computational expertise. It contains pre-defined workflows for image segmentation, object classification, counting and tracking. Users adapt the workflows to the problem at hand by interactively providing sparse training annotations for a nonlinear classifier. ilastik can process data in up to five dimensions (3D, time and number of channels). Its computational back end runs operations on-demand wherever possible, allowing for interactive prediction on data larger than RAM. Once the classifiers are trained, ilastik workflows can be applied to new data from the command line without further user interaction. We describe all ilastik workflows in detail, including three case studies and a discussion on the expected performance. SP - 1 Y1 - 2019/09// UR - https://archiv.ub.uni-heidelberg.de/volltextserver/28283/ KW - deep learning in microscopy KW - machine learning KW - image analysis TI - ilastik: interactive machine learning for (bio)image analysis ER -