eprintid: 28283 rev_number: 35 eprint_status: archive userid: 5130 dir: disk0/00/02/82/83 datestamp: 2020-05-07 12:00:58 lastmod: 2020-06-15 12:56:47 status_changed: 2020-05-07 12:00:58 type: article metadata_visibility: show creators_name: Berg, Stuart creators_name: Kutra, Dominik creators_name: Kroeger, Thorben creators_name: Straehle, Christoph N. creators_name: Kausler, Bernhard X. creators_name: Haubold, Carsten creators_name: Schiegg, Martin creators_name: Ales, Janez creators_name: Beier, Thorsten creators_name: Rudy, Markus creators_name: Eren, Kemal creators_name: Cervantes, Jaime I creators_name: Xu, Buote creators_name: Beuttenmueller, Fynn creators_name: Wolny, Adrian creators_name: Zhang, Chong creators_name: Koethe, Ullrich creators_name: Hamprecht, Fred A. creators_name: Kreshuk, Anna title: ilastik: interactive machine learning for (bio)image analysis subjects: ddc-004 subjects: ddc-570 subjects: ddc-600 divisions: i-708000 divisions: i-708070 divisions: i-850800 keywords: deep learning in microscopy, machine learning, image analysis cterms_swd: Deep learning cterms_swd: Mikroskopie cterms_swd: Maschinelles Lernen cterms_swd: Bildanalyse cterms_swd: Software abstract: We present ilastik, an easy-to-use interactive tool that brings machine-learning-based (bio)image analysis to end users without substantial computational expertise. It contains pre-defined workflows for image segmentation, object classification, counting and tracking. Users adapt the workflows to the problem at hand by interactively providing sparse training annotations for a nonlinear classifier. ilastik can process data in up to five dimensions (3D, time and number of channels). Its computational back end runs operations on-demand wherever possible, allowing for interactive prediction on data larger than RAM. Once the classifiers are trained, ilastik workflows can be applied to new data from the command line without further user interaction. We describe all ilastik workflows in detail, including three case studies and a discussion on the expected performance. date: 2019-09 publisher: Nature Publishing Group id_scheme: DOI ppn_swb: 1698533136 own_urn: urn:nbn:de:bsz:16-heidok-282831 language: eng bibsort: BERGSTUARTILASTIKINT201909 full_text_status: public publication: Nature Methods volume: 2019 number: 16 place_of_pub: London, New York pagerange: 1-9 issn: 1548-7105 (Online-Ausg.), 1548-7091 (Druck-Ausg.) citation: Berg, Stuart ; Kutra, Dominik ; Kroeger, Thorben ; Straehle, Christoph N. ; Kausler, Bernhard X. ; Haubold, Carsten ; Schiegg, Martin ; Ales, Janez ; Beier, Thorsten ; Rudy, Markus ; Eren, Kemal ; Cervantes, Jaime I ; Xu, Buote ; Beuttenmueller, Fynn ; Wolny, Adrian ; Zhang, Chong ; Koethe, Ullrich ; Hamprecht, Fred A. ; Kreshuk, Anna (2019) ilastik: interactive machine learning for (bio)image analysis. Nature Methods, 2019 (16). pp. 1-9. ISSN 1548-7105 (Online-Ausg.), 1548-7091 (Druck-Ausg.) document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/28283/7/Berg_ilastik_2020.pdf