<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Unraveling the mysteries of the plant cell wall: A characterization of three novel proteins related to cell wall signaling"^^ . "The field of plant cell wall signaling is one that has seen tremendous growth in the last few decades. One result of this has been the identification of many uncharacterized proteins putatively involved in the cell wall signaling network. This thesis outlines the initial characterization of three previously unstudied genes of interest in the model organism Arabidopsis thaliana which are loosely tied together by their apparent links, either directly or indirectly, with cell wall signaling. The first gene investigated, named PHYTOSULFOKINE LIKE 1 (PSKL1), is what seems to be a potential preproprotein with possible links to Phytosulfokine (PSK) singling and RECEPTOR-LIKE-PROTEIN 44 (RLP44). PSKL1 was found to contain two of the YIYTQ amino acid motifs found in PSK genes and to affect vascular cell identity. This was shown by an increase in the average number of metaxylem cells in the roots of pskl1 mutant seedlings, a phenotype that was rescued back to wildtype by exogenous PSK treatment. The inverse of this phenotypic trend was observed in a mutant line where both PSK motifs in the gene remained intact, suggesting that PSKL1 might function as more than a possible source of mature PSK’s. The second gene investigated is a member of the F-Box/RNI Like family. This gene was identified in a forward genetic screen looking for suppressors of the RLP44ox phenotype, where it emerged as the most likely candidate for a Brassinosteroid (BR) signaling independent RLP44ox suppressor. Generation of a f-box/rni like line in the RLP44ox background using the CRISPR/Cas9 system recapitulated the suppression phenotype, supporting its role as the causative mutant gene in the original forward genetic screen. Use of a F-Box/RNI Like:RFP line showed F-Box/RNI Like to be localized to the cytosol and a lack of colocalization with RLP44:GFP, suggesting that it doesn’t directly associate with RLP44. The third gene investigated is a receptor like protein named RECEPTOR-LIKE- PROTEIN 46 (RLP46), which has a high degree of genetic conservation throughout the plant kingdom. Available data showed highest RLP46 expression in mature root tissue, and a strong upregulation of expression upon exposure to the elicitor elf18, suggesting RLP46 might have a role in plant innate immune response. The CRISPR/Cas9 system was used to generate a rlp46 mutant line which was tested for biotic and abiotic stress phenotypes. rlp46 at first showed a possible NaCl resistance phenotype, but repetitions of the experiment gave conflicting results. There was however a subtle but consistent phenotype of resistance to elicitor induced growth inhibition in the rlp46 line. Co-immunoprecipitation and Western Blot showed evidence for an association between RLP46 and SUPPRESSOR-OF-BIR 1 (SOBIR1), providing a strong clue for how RLP46 might interact with the plant immune response signaling network. The experiments outlined in this thesis proved successful in characterizing these three proteins and provide a firm foundation for future research."^^ . "2020" . . . . . . . "John Brevoort"^^ . "Walden"^^ . "John Brevoort Walden"^^ . . . . . . "Unraveling the mysteries of the plant cell wall: A characterization of three novel proteins related to cell wall signaling (PDF)"^^ . . . "John Walden PhD Thesis Final Draft reduced A.pdf"^^ . . . "Unraveling the mysteries of the plant cell wall: A characterization of three novel proteins related to cell wall signaling (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Unraveling the mysteries of the plant cell wall: A characterization of three novel proteins related to cell wall signaling (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Unraveling the mysteries of the plant cell wall: A characterization of three novel proteins related to cell wall signaling (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Unraveling the mysteries of the plant cell wall: A characterization of three novel proteins related to cell wall signaling (Other)"^^ . . . . . . "small.jpg"^^ . . . "Unraveling the mysteries of the plant cell wall: A characterization of three novel proteins related to cell wall signaling (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #28354 \n\nUnraveling the mysteries of the plant cell wall: A characterization of three novel proteins related to cell wall signaling\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . . . "580 Pflanzen (Botanik)"@de . "580 Botanical sciences"@en . .