eprintid: 28401
rev_number: 25
eprint_status: archive
userid: 5204
dir: disk0/00/02/84/01
datestamp: 2020-06-05 14:48:53
lastmod: 2020-06-09 13:13:08
status_changed: 2020-06-05 14:48:53
type: preprint
metadata_visibility: show
creators_name: Cheng, Jingdong
creators_name: Baßler, Jochen
creators_name: Fischer, Paulina
creators_name: Lau, Benjamin
creators_name: Kellner, Nikola
creators_name: Kunze, Ruth
creators_name: Griesel, Sabine
creators_name: Kallas, Martina
creators_name: Berninghausen, Otto
creators_name: Strauß, Daniela
creators_name: Beckmann, Roland
creators_name: Hurt, Ed
title: Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration
subjects: 570
divisions: 711000
abstract: The ‘birth’ of the eukaryotic ribosome is preceded by RNA folding and processing reactions that depend on assembly factors and snoRNAs. The 90S (SSU-processome) is the earliest pre-ribosome structurally analyzed, which was suggested to assemble stepwise along the growing pre-rRNA from 5’>3’, but this directionality may not be accurate. Here, by analyzing the structure of series of novel 90S assembly intermediates isolated from Chaetomium thermophilum, we discover a reverse order of 18S rRNA subdomain incorporation. This revealed that large parts of the 18S rRNA 3’ and central domains assemble first into the 90S, before the 5’ domain is stably integrated. This final incorporation depends on a physical contact between a heterotrimer Enp2-Bfr2-Lcp1 recruited to the flexible 5’ domain and Kre33, which reconstitutes the Kre33-Enp-Brf2-Lcp5 module on the compacted 90S pre-ribosome. Keeping the 5’ domain temporarily segregated from the 90S scaffold could provide an extra time to complete the multifaceted 5’ domain folding, which depends on a distinct set of snoRNAs and processing factors.
date: 2019-09
publisher: Elsevier ; Cell Press
id_scheme: DOI
id_number: 10.11588/heidok.00028401
fp7_project_id: ERC ADG 781741 GLOWSOME
official_url: https://doi.org/10.1016/j.molcel.2019.06.032
ppn_swb: 1700202901
own_urn: urn:nbn:de:bsz:16-heidok-284013
language: eng
bibsort: CHENGJINGDTHERMOPHIL201909
full_text_status: public
place_of_pub: New York ; Cambridge, Mass.
citation:   Cheng, Jingdong ; Baßler, Jochen ; Fischer, Paulina ; Lau, Benjamin ; Kellner, Nikola ; Kunze, Ruth ; Griesel, Sabine ; Kallas, Martina ; Berninghausen, Otto ; Strauß, Daniela ; Beckmann, Roland ; Hurt, Ed  (2019) Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration.  [Preprint]     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/28401/1/Kre33-Noc4-90S-manuscript.pdf