eprintid: 28584
rev_number: 15
eprint_status: archive
userid: 5256
dir: disk0/00/02/85/84
datestamp: 2020-09-22 06:45:05
lastmod: 2020-09-22 12:09:10
status_changed: 2020-09-22 06:45:05
type: doctoralThesis
metadata_visibility: show
creators_name: Yang, Fan
title: Re-engineering a Nanoparticle Human Papillomavirus Prophylactic Vaccine Antigen Based on the Minor Capsid Protein L2
subjects: ddc-570
divisions: i-911600
divisions: i-911700
adv_faculty: af-05
abstract: Human papillomavirus (HPV) is the main cause of cervical cancer, which represents a major global health burden. Despite their success, current commercial virus-like particle vaccines have certain limitations, in particular the virus type-restricted antibody responses and the need for intact cooling chains in vaccine distribution. Here, we employed self-assembling nanoparticles that display different epitopes of the HPV minor capsid protein L2. Presenting the L2 epitopes on a scaffold provided by thioredoxin of the archaeon Pyrococcus furiosus (Pf Trx) in combination with a nanoparticle super-scaffold of Pyrococcus furiosus ferritin and encapsulin results in an antigen able to induce broadly protective antibody response against oncogenic and non-oncogenic HPV types in a small animal model. Moreover, in mice this protection could last one year after immunization. Considering of the safety of the vaccine antigen scaffold, we confirmed there was no cross-reactivity of antibodies induce against human thioredoxin or human ferritin. Thus, the nanoparticle vaccine induces robust titers of neutralizing antibodies, which are broadly reactive and persistent also providing protection in an in vivo mouse challenge model.
date: 2020
id_scheme: DOI
id_number: 10.11588/heidok.00028584
ppn_swb: 1733532714
own_urn: urn:nbn:de:bsz:16-heidok-285846
date_accepted: 2020-07-23
advisor: HASH(0x55a9a65b0130)
language: eng
bibsort: YANGFANREENGINEER2020
full_text_status: public
place_of_pub: Heidelberg
citation:   Yang, Fan  (2020) Re-engineering a Nanoparticle Human Papillomavirus Prophylactic Vaccine Antigen Based on the Minor Capsid Protein L2.  [Dissertation]     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/28584/1/thesis%20-Fan%20Yang.pdf