<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Bottom-up Assembly of Functional Extracellular Vesicles\r\n– Implications for Synthetic Biology and Biomedical\r\nApplications"^^ . "Formation of lipid-based compartments is a distinguishing feature of eukaryotic\r\nlife forms. These compartments play a crucial role in orchestrating independent\r\nand self-contained metabolic, signalling or synthesis processes. Moreover, cellderived\r\nlipid compartments, like extracellular vesicles (EVs), have been shown to be\r\nessential for intercellular signalling and are involved in a wide variety of disease states.\r\nAlthough attaining a holistic understanding of EV-based communication is a compelling\r\ngoal, the extensive molecular and structural complexity of these vesicles as well as a lack\r\nof reliable EV isolation techniques, have impaired detailed mechanistic insights. Inspired\r\nby bottom-up synthetic biology principles, the central goal of my interdisciplinary\r\nresearch was the development of a bio-inspired EV model system, which serves as a\r\nplatform to study EV-based intercellular signalling and empowers novel EV-inspired\r\ntherapeutics.\r\nIn this thesis, I present two major methodologies developed for the controlled\r\nhigh-throughput assembly of synthetic vesicles. First, I describe a droplet-based\r\nmicrofluidic approach for the production of giant unilamellar vesicles (GUVs) with wellcontrolled\r\nbiophysical and biochemical properties. I report on systematic investigations\r\nof GUV interactions with living cells and present concepts on how fine-tuning of the\r\nvesicles surface characteristics can be applied for targeted cellular delivery of\r\nmacromolecular cargos. Moreover, I show how these vesicles can be reconceptualised as\r\nsynthetic organelles, functioning within living cells and providing them with synthetic\r\nfunctionalities. Based on these fundamental characterizations, in the second part of my\r\nthesis, I present a complementary and quantitative approach for the sequential bottom-up\r\nassembly of fully synthetic EVs (fsEVs). To exemplify the application of fsEVs for new\r\ntherapeutic concepts, I show that they exert analogous functionalities to naturally\r\noccurring wound healing EVs. Furthermore, by combining the fsEV technology with\r\nwhole-transcriptome analysis, I systematically decode the synergistic functionalities\r\nbetween individual EV components. This approach enabled me to perform an analytical\r\ndissection of the associated EV signalling processes mediated by tetraspanin proteins.\r\nBioinspired and biocompatible synthetic compartments with precisely controllable\r\nbiophysical and biochemical properties are desirable tools for a wide range of living and\r\nsynthetic cells research. This study makes it tempting to view EV-like compartments in a broader perspective. For example, they have great application potential as on-demand\r\ndrug delivery systems, paving the way for hitherto impossible approaches towards\r\nadministration of advanced cargos such as microparticles, viruses or synthetic organelles.\r\nMoreover, I anticipate that the highly controlled assembly of fsEVs will provide a robust\r\nframework for innovative therapeutic applications of bottom-up assembled synthetic\r\nbiological modules and will additionally allow for new insights into fundamental EVrelated\r\nprinciples that govern cellular communication."^^ . "2021" . . . . . . . "Oskar"^^ . "Staufer"^^ . "Oskar Staufer"^^ . . . . . . "Bottom-up Assembly of Functional Extracellular Vesicles\r\n– Implications for Synthetic Biology and Biomedical\r\nApplications (PDF)"^^ . . . "PhDThesisOskarStauferCompressed.pdf"^^ . . . "Bottom-up Assembly of Functional Extracellular Vesicles\r\n– Implications for Synthetic Biology and Biomedical\r\nApplications (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Bottom-up Assembly of Functional Extracellular Vesicles\r\n– Implications for Synthetic Biology and Biomedical\r\nApplications (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Bottom-up Assembly of Functional Extracellular Vesicles\r\n– Implications for Synthetic Biology and Biomedical\r\nApplications (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Bottom-up Assembly of Functional Extracellular Vesicles\r\n– Implications for Synthetic Biology and Biomedical\r\nApplications (Other)"^^ . . . . . . "small.jpg"^^ . . . "Bottom-up Assembly of Functional Extracellular Vesicles\r\n– Implications for Synthetic Biology and Biomedical\r\nApplications (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #28803 \n\nBottom-up Assembly of Functional Extracellular Vesicles \n– Implications for Synthetic Biology and Biomedical \nApplications\n\n" . "text/html" . . . "000 Allgemeines, Wissenschaft, Informatik"@de . "000 Generalities, Science"@en . .