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primary topic: Landscape and functions of RNA localization in the Drosophila follicular epithelium

Landscape and functions of RNA localization in the Drosophila follicular epithelium
type: Article
abstract: The mechanisms and roles underlying the phenomenon of RNA localization in differentiated tissues are poorly characterized. Drosophila follicular epithelium represents a good model to study RNA localization in the context of an adult tissue, for several reasons. First, it expresses the dynein/BicD/Egl RNA localization machinery, which transports RNAs to the apical domain. Second, it is genetically tractable, offering the possibility to generate mutant clones for a gene of interest in a mosaic manner. So far, only a handful of mRNAs showing a subcellular localization pattern in the follicular epithelium have been reported, and the extent and significance of the phenomenon are not known. To address this, I applied a spatial transcriptomics approach to identify the mRNAs that show apical or basal localization patterns in this tissue. Laser-capture microdissection (LCM) followed by RNA-seq and bioinformatic analysis allowed the identification of 520 differentially enriched mRNAs in either the apical or the basal domain. Gene Ontology (GO) enrichment analysis showed a functional compartmentalization of mRNAs in different cellular domains, suggesting that mRNAs encoding components of the same organelle or pathway might be locally translated. Next, I investigated the mechanisms that govern apical and basal RNA localization in the follicular epithelium, by knocking down components of known microtubule (MT) minus end- and plus end-directed RNA transport. I found that apical RNA localization is achieved mainly by the activity of the dynein/BicD/Egl RNA transport machinery, but that at least one mRNA is localized apically by another MT-based mechanism. On the other hand, basal RNA localization is dependent on kinesin-1 and, in some cases, is regulated by the deposition of the Exon Junction Complex (EJC), reminiscent of the mechanisms that underlie oskar RNA localization to the posterior pole. Interestingly, disrupting kinesin-1 by Khc RNAi causes basal mRNAs to localize apicall…
date: 2021
author list: authors
type: EPrint
type: DoctoralThesisEPrint
is part of: repository
has document: Landscape and functions of RNA localization in the Drosophila follicular epithelium (PDF)
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subject: 570 Biowissenschaften, Biologie

Landscape and functions of RNA localization in the Drosophila follicular epithelium (PDF)
type: Document
has file: final_thesis.pdf
has part: final_thesis.pdf

Lucia Cassella
type: Person
given name: Lucia
family name: Cassella

Landscape and functions of RNA localization in the Drosophila follicular epithelium (Other)
type: Document
is version of: Landscape and functions of RNA localization in the Drosophila follicular epithelium (PDF)
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Landscape and functions of RNA localization in the Drosophila follicular epithelium (Other)
type: Document
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Landscape and functions of RNA localization in the Drosophila follicular epithelium (Other)
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Landscape and functions of RNA localization in the Drosophila follicular epithelium (Other)
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Landscape and functions of RNA localization in the Drosophila follicular epithelium (Other)
type: Document
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primary topic: Landscape and functions of RNA localization in the Drosophila follicular epithelium

570 Biowissenschaften, Biologie
type: Concept
preferred label: 570 Life sciences @en