<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Entrainment of coupled, phase-shifted signaling oscillations in the presomitic mesoderm"^^ . "Synchronization is everywhere in nature. It is an emergent property arising in systems of interacting oscillatory entities, regardless if these entities are physical (e.g. in an electrical circuit) or biological (e.g. a group of fireflies). In vertebrate embryos, synchronization of intracellular signaling oscillations regulates the precise and periodic formation of somites, the precursors of vertebrae. In this system, oscillations are coordinated between neighbors via intercellular coupling, and such coordination results in a phase shift between oscillations, giving the impression of a spatiotemporal wave pattern travelling through the presomitic mesoderm (PSM) from posterior to anterior. Where this wave arrests at the anterior PSM, a new somite forms. The timing of such an event is mediated by the segmentation clock, an elaborate molecular signaling network between Notch, Wnt, and FGF signaling. \r\n\r\nWhile there are numerous studies focusing on the molecular details underlying such spatiotemporal regulation from the bottom-up, research delving into (1) the nature and mechanism of its synchronization and (2) its impact on tissue patterning during embryogenesis remains limited. To address this, we thus instead focus on a principles-based, top-down, approach. Accordingly, we recently developed a microfluidics-based experimental platform allowing entrainment of the signaling oscillations in the PSM to periodic pulses of signaling modulators, leveraging fundamental entrainment principles that have also been studied in other complex physical and biological oscillatory systems (e.g. the circadian clock). \r\n\r\nIn this current research, we use such experimental platform to map Arnold tongues, to systematically control both the period and the phase of oscillations in the PSM, and to precisely modulate the segmentation clock. We report how the systems-level entrainment behavior of oscillations in an embryonic tissue follows dynamical systems theory, despite its complexity. Furthermore, we uncover- and elaborate on a peculiar behavior in our system (i.e. emergence of a period gradient even upon tissue-level entrainment), providing insight into the nature of the underlying oscillatory network in the PSM. This finding has enabled us to generate testable hypotheses about the importance of the period gradient for the processing of spatiotemporal cues and proper tissue patterning. Moreover, experiments with intact embryonic tissue have allowed us to link modulation of the segmentation clock and its consequences on patterning of the PSM. We specifically record observations in apparent contradiction with traditional interpretations of a well-known model of periodic patterning during somitogenesis (i.e. the classical clock and wavefront model). Our observations instead support the proposition that the oscillatory dynamics encode both the timing and spacing of somite formation.\r\n\r\nMore generally, we here highlight the power of our experimental approach to precisely control the period and phase of a complex spatially-extended system of coupled and phase-shifted oscillations in an embryonic tissue, which had not been possible before using genetics and pharmacological intervention. We hope this research provides further experimental evidence of the universality of fundamental entrainment principles, and offers an alternative top-down approach to the study of synchronization of biological oscillations in embryonic development."^^ . "2021" . . . . . . . "Paul Gerald Layague"^^ . "Sanchez"^^ . "Paul Gerald Layague Sanchez"^^ . . . . . . "Entrainment of coupled, phase-shifted signaling oscillations in the presomitic mesoderm (PDF)"^^ . . . "PGLSanchez-EMBL_Dissertation.pdf"^^ . . . "Entrainment of coupled, phase-shifted signaling oscillations in the presomitic mesoderm (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Entrainment of coupled, phase-shifted signaling oscillations in the presomitic mesoderm (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Entrainment of coupled, phase-shifted signaling oscillations in the presomitic mesoderm (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Entrainment of coupled, phase-shifted signaling oscillations in the presomitic mesoderm (Other)"^^ . . . . . . "small.jpg"^^ . . . "Entrainment of coupled, phase-shifted signaling oscillations in the presomitic mesoderm (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #29209 \n\nEntrainment of coupled, phase-shifted signaling oscillations in the presomitic mesoderm\n\n" . "text/html" . . . "500 Naturwissenschaften und Mathematik"@de . "500 Natural sciences and mathematics"@en . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .