<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Origination, Monoclonality and Evolution of the\r\nMarbled Crayfish Genome Procambarus virginalis"^^ . "The parthenogenetic marbled crayfish (Procambarus virginalis) sparked interest within\r\nthe scientific community due to its unique features. Its polyploid and monoclonal genome, high environmental adaptability and phenotypic diversity made the marbled crayfish\r\na suitable laboratory model for genomics, epigenetics and ecology research.\r\nThe previously established marbled crayfish genome sequence of 3.5 Gbp represents\r\na highly fragmented draft assembly. Initial comparative genomic analyses resulted in confirmation of P. virginalis genome origination from the sexually reproducing freshwater crayfish P. fallax. However, in-depth genomic analysis and interspecies genome comparisons\r\nrequire further refinement of the fragmented genome reference of the marbled crayfish.\r\nIn this PhD thesis, the first refinement of the marbled crayfish genome has been performed with application of the PacBio Single Molecule Real Time (SMRT) sequencing\r\ntechnology. The new and improved genome assembly of the marbled crayfish resulted in\r\n3.7 Gbp of sequence length and an N50 of 144kb. The refined genome assembly enabled\r\nsearching parental haplotypes and understanding species origination. The absence of\r\nevidence for loss of heterozygosity in the various monoclonal marbled crayfish generations\r\nsuggests the lack of recombination process during oogenesis. Thus, marbled crayfish suggest to be apomictic parthenogens which are characterized by generating identical copies\r\nof the maternal genotype. Moreover, despite of the limited genome variability, monoclonal\r\nmarbled crayfish genomes consisted of population-specific genetic polymorphisms within\r\nthe global population. Comparative genomic analysis between geographically distant populations resulted in the identification of population-specific mutational signatures. The\r\ncalculation of genomic variability of marbled crayfish from the growing population in Lake\r\nReilingen allowed to estimate population dynamics. Thus, the population in Lake Reilingen\r\ndemonstrates a rapid growth, following the density-independent exponential model.\r\nThis PhD thesis provides fundamental insights into marbled crayfish research, particularly via making use of an improved genome assembly for comparative genomic analyses,\r\nepigenetic studies, and for research on the evolution and genomic adaptation to asexuality."^^ . "2021" . . . . . . . "Olena"^^ . "Maiakovska"^^ . "Olena Maiakovska"^^ . . . . . . "Origination, Monoclonality and Evolution of the\r\nMarbled Crayfish Genome Procambarus virginalis (PDF)"^^ . . . "PhDThesis.pdf"^^ . . . "Origination, Monoclonality and Evolution of the\r\nMarbled Crayfish Genome Procambarus virginalis (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Origination, Monoclonality and Evolution of the\r\nMarbled Crayfish Genome Procambarus virginalis (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Origination, Monoclonality and Evolution of the\r\nMarbled Crayfish Genome Procambarus virginalis (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Origination, Monoclonality and Evolution of the\r\nMarbled Crayfish Genome Procambarus virginalis (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Origination, Monoclonality and Evolution of the\r\nMarbled Crayfish Genome Procambarus virginalis (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #29661 \n\nOrigination, Monoclonality and Evolution of the \nMarbled Crayfish Genome Procambarus virginalis\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .