<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Identification and characterization of genes involved in stilbene biosynthesis and modification in Vitis vinifera"^^ . "Stilbenes are plant secondary metabolites from the polyphenols group found in 72 unrelated plant species. Among those plants is Vitis vinifera, an important crop plant in wine regions all across the world. The natural functions of stilbenes include the reaction to stress factors like UV-radiation and mechanical injury or defense reactions against bacterial, fungal or other pathogens. One of the most problematic pathogens in the wine regions in Germany is Plasmopara viticola, causing downy mildew, which leads to great crop losses. Aside from that, stilbenes have gotten attention in the recent years for their potent health beneficial properties. A multitude of studies, mainly pre-clinical trials, have indicated that stilbenes can delay aging parameters, help in the prevention of diseases including cancer or diabetes and are effective against cardiovascular diseases. \r\nWhile much attention is given to the functions of stilbenes, both in planta and in the medical or nutritional supplement areas, and the structures and occurrences of stilbenes are mostly known, the biosynthetic pathway leading to this wide variety of modified stilbenes remains largely unknown. The precursors of the stilbenes derive from the phenylpropanoid pathway, where the stilbene synthase, as first dedicated enzyme of the pathway produces trans-resveratrol. Apart from that, only one other enzyme, a resveratrol-O-methyl transferase and the two transcription factors (TFs) VvMYB14 and VvMYB15 are known and characterized. Other enzymes, for example for glycosylation, polymerization or other modification reactions remain yet to be found, as do dedicated transport proteins. \r\nDifferent V. vinifera tissues overexpressing VvMYB15, one of the two TFs that were shown to be important regulators of the stilbene biosynthesis pathway, were used to run two microarrays prior to this project. From this database, candidate genes with potentially important predicted functions were identified by their upregulation. The selected genes were then filtered by the means of in silico analysis and promoter induction assays before the correlation of their gene expression with the content of modified stilbenes, expected to be produced by their reaction, was investigated. This was done in V. vinifera berries of different developmental stages as well as leaf discs infected with P. viticola and allowed for the closer connection of several candidate genes with their expected products, while also giving valuable insight into the potential role of the stilbenes, and thus the genes, during development or in a defense against infection. In the last part of this thesis, the most promising candidate genes, three glycosyltransferases and a laccase, underwent closer biochemical characterization by expression in bacteria, yeast or tobacco and subsequent enzymatic assay or direct HPLC or UHPLC/MS analysis. While the full characterization of the candidate genes remains to be completed, promising results for the glycosyltransferases were obtained concerning the metabolization of resveratrol and other substrates and for the involvement of the laccase and viniferin in defense reactions."^^ . "2021" . . . . . . . "Tobias"^^ . "Ziegler"^^ . "Tobias Ziegler"^^ . . . . . . "Identification and characterization of genes involved in stilbene biosynthesis and modification in Vitis vinifera (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Identification and characterization of genes involved in stilbene biosynthesis and modification in Vitis vinifera (PDF)"^^ . . . "Dissertation Tobias Ziegler.pdf"^^ . . . "Identification and characterization of genes involved in stilbene biosynthesis and modification in Vitis vinifera (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Identification and characterization of genes involved in stilbene biosynthesis and modification in Vitis vinifera (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Identification and characterization of genes involved in stilbene biosynthesis and modification in Vitis vinifera (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Identification and characterization of genes involved in stilbene biosynthesis and modification in Vitis vinifera (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #30000 \n\nIdentification and characterization of genes involved in stilbene biosynthesis and modification in Vitis vinifera\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . . . "580 Pflanzen (Botanik)"@de . "580 Botanical sciences"@en . .