<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Investigating the impact of cell wall dynamics\r\non secondary growth regulation"^^ . "The morphology of plants can be highly adaptive due to the plasticity governed by\r\npostembryonic development. In plants, stem cell populations, called meristems are\r\nmaintained through the entire life and enable lifelong development. Longitudinal growth\r\nis realized by the meristematic activity in the apices of the shoot and root, while lateral\r\ngrowth is enabled by the activity of the vascular cambium that is able to produce\r\nspecialized tissues in a bifacial manner. Initially, the fascicular cambium is active solely\r\nin vascular bundles. At a later stage of development, the fascicular cambium extends\r\nand cells in the region between vascular bundles – the so-called interfascicular region\r\n– start to divide, cumulating in the post-embryonic establishment of the vascular\r\ncambium. Its formation is potentially based on de-differentiation of differentiated cells.\r\nHowever, how exactly this process is initiated is still poorly understood.\r\nHere, I used the process of vascular cambium formation, as a unique model to study\r\ncell fate change. To control the formation of vascular cambium, I used a genetic tool\r\nthat allowed me to induce auxin biosynthesis in a cell type-specific manner. Strikingly,\r\ninduction of auxin biosynthesis in fully differentiated starch sheath cells provoked a cell\r\nfate change and initiated the formation of interfascicular cambium. Based on detailed\r\nanalysis of interfascicular cambium formation, nuclear auxin signaling is shown to be\r\ncritical for this process. Furthermore, I investigated the role of cell wall during vascular\r\ncambium formation, as plant cells are immobilized by cell walls – a rigid type of\r\nextracellular matrix. The thereby fixed position of the cell can contain important\r\ninformation for cell fate determination. Interestingly, extensive cell wall remodeling\r\ntakes place during cambium formation, as revealed by immunohistochemical\r\nquantification of extensin abundance and pectin modifications. Genome-wide\r\ntranscriptional profiling upon auxin-induced interfascicular cambium formation further\r\nsupports this result, as genes related to cell wall remodeling were disproportionately\r\nrepresented among differentially expressed genes. Finally, I demonstrated the\r\nimportance of cell wall remodeling during interfascicular cambium formation by blocking\r\nauxin induced interfascicular cambium formation via inhibiting cell wall remodeling.\r\nThe reported change in cell wall composition during vascular cambium formation may\r\ncause a change in the elasticity of the cell wall, which might in turn be a prerequisite for\r\nlateral growth. To quantitatively measure the elasticity of cell walls, I established\r\nBrillouin microscopy in our lab and investigated cell wall properties depending on their\r\norientation and localization. I was able to show that Brillouin microscopy is a suitable\r\nmethod to further investigate cell wall mechanics and its impact in the field of plant\r\nscience."^^ . "2021" . . . . . . . "Theresa"^^ . "Schlamp"^^ . "Theresa Schlamp"^^ . . . . . . "Investigating the impact of cell wall dynamics\r\non secondary growth regulation (PDF)"^^ . . . "Dissertation_Theresa Schlamp.pdf"^^ . . . "Investigating the impact of cell wall dynamics\r\non secondary growth regulation (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Investigating the impact of cell wall dynamics\r\non secondary growth regulation (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Investigating the impact of cell wall dynamics\r\non secondary growth regulation (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Investigating the impact of cell wall dynamics\r\non secondary growth regulation (Other)"^^ . . . . . . "small.jpg"^^ . . . "Investigating the impact of cell wall dynamics\r\non secondary growth regulation (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #30034 \n\nInvestigating the impact of cell wall dynamics \non secondary growth regulation\n\n" . "text/html" . . . "500 Naturwissenschaften und Mathematik"@de . "500 Natural sciences and mathematics"@en . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .