<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "PNIPAM-containing droplets: The route towards an artificial cytoskeleton assembly based on synthetic and natural components"^^ . "Although cells represent the smallest building blocks of life, they are already exhibiting a high level of complexity. It is therefore not surprising that researchers tend to utilize simplified systems for investigating, but also reconstituting, cellular processes in artificially constructed cells. The conception of those artificial cells can be done using bottom-up biological approaches. Currently, the reconstitution of cellular process is\r\nachieved using natural or nature-derived components like the actomyosin-complex for the reconstitution of cellular motility. Although a reduction of complexity can be accomplished in this way, natural systems still require specialized conditions and sophisticated buffers.\r\nUltimately, the reduction of complexity can only be realized by the reconstitution of cellular processes using completely synthetic materials.\r\nIn this thesis, a completely artificial cytoskeleton based on thermo-responsive poly(N-isopropylacrylamide) (PNIPAM) to trigger motility of synthetic cells was established. To this end, a PNIPAM-based composite material containing gold nanorods was generated and its physiochemical behavior was characterized. The composite material was introduced and assembled inside water-in-oil emulsion droplets that have been stabilized by either a custom-made PNIPAM-containing surfactant (PNS) or a commercially available fluorosurfactant (CS). Besides the reversibility of the PNIPAM phase transition in bulk and droplet-based synthetic cells, the on-demand inducible deformation of droplets due to this phase transition was shown. Additionally, droplet\r\nmigration was triggered using asymmetric PNIPAM-containing droplets in combination with fluorophilic-coated surfaces. The production of those cell polarization-mimicking\r\ndroplets was achieved via targeted fusion of stable and unstable droplets during polymerization. Following the assessment of PNIPAM-mediated droplet motility, for the\r\nfirst time, the artificial cytoskeleton material was combined with the natural cytoskeleton protein actin by a sequential pico-injection approach thus elucidating the biocompatibility of the presented system.\r\nHerein, a versatile PNIPAM-based artificial cytoskeleton for synthetic cell applications was designed and implemented.\r\nThe control over the PNIPAM volume transition processes allowed to achieve the motility of the droplet-based synthetic cells by their dynamic deformation. The biocompatibility of the system by combining natural and synthetic cytoskeleton components could be shown.\r\nThe tolerance of the PNIPAM system also towards specialized buffer systems, might expand the bandwidth of the bottom-up synthetic biology tool kit, enhancing the assembly of hybrid cellular modules. Therefore, the developed approach represents an important milestone in the development of bottom-up synthetic biology applications."^^ . "2021" . . . . . . . "Désirée"^^ . "Sauter"^^ . "Désirée Sauter"^^ . . . . . . "PNIPAM-containing droplets: The route towards an artificial cytoskeleton assembly based on synthetic and natural components (Other)"^^ . . . . . . "medium.jpg"^^ . . . "PNIPAM-containing droplets: The route towards an artificial cytoskeleton assembly based on synthetic and natural components (Other)"^^ . . . . . . "small.jpg"^^ . . . "PNIPAM-containing droplets: The route towards an artificial cytoskeleton assembly based on synthetic and natural components (Other)"^^ . . . . . . "preview.jpg"^^ . . . "PNIPAM-containing droplets: The route towards an artificial cytoskeleton assembly based on synthetic and natural components (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "PNIPAM-containing droplets: The route towards an artificial cytoskeleton assembly based on synthetic and natural components (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "PNIPAM-containing droplets: The route towards an artificial cytoskeleton assembly based on synthetic and natural components (PDF)"^^ . . . "DissertationDesireeSauter.pdf"^^ . . "HTML Summary of #30507 \n\nPNIPAM-containing droplets: The route towards an artificial cytoskeleton assembly based on synthetic and natural components\n\n" . "text/html" . . . "540 Chemie"@de . "540 Chemistry and allied sciences"@en . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .