…
comment: The repository administrator has not yet configured an RDF license.
primary topic: Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse

Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse
type: Article
abstract: Two-photon microscopy is currently the technique of choice for deep imaging in scattering, opaque specimen such as the in-vivo mouse, due to inherent optical sectioning and longer wavelength excitation light, which is generally less effected by scattering. However, the maximum penetration depth of two-photon microscopes is fundamentally limited by the on-set of out-of-focus fluorescence near the surface with increasing excitation power, which for the mammalian brain prevents imaging beyond ~1mm. Three-photon excitation fundamentally improves the depth limit due to a significantly increased signal-to-background ratio at depth and longer wavelength excitation. Unfortunately, optical aberrations stemming from the optical system and inhomogeneities within the sample lead to a degradation of resolution and contrast and loss of signal intensity at depth. However, aberrations can be corrected and near diffraction limited resolution recovered with so-called adaptive optics strategies. While two-photon microscopy has been combined with adaptive optics to correct for aberrations, very fine, sub- micron structures such as spines in the mouse brain are difficult to resolve with current methods in deep cortical or even sub-cortical brain regions. To tackle this challenge, in my PhD work I developed a custom three-photon microscope with integrated adaptive optics to increase the practical imaging depth and resolution for non-invasive in-vivo imaging of mouse tissue with the main focus on neuroscience application. In particular, I have designed and build a custom multi-photon microscope based on 1300nm excitation and shown its capability to image GFP-labeled neuron somata and even small structures such as dendritic branches up to a depth of 1.2mm in the intact mouse brain, which is among the best achievements demonstrated so far in the literature. At such large tissue depths, however, heart pulsation leads to brain motion and thus to intra-frame artefacts which prevent frame avera…
date: 2021
author list: authors
type: EPrint
type: DoctoralThesisEPrint
is part of: repository
has document: Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (PDF)
has document: Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (Other)
subject: 000 Allgemeines, Wissenschaft, Informatik
subject: 500 Naturwissenschaften und Mathematik
subject: 530 Physik
subject: 570 Biowissenschaften, Biologie

Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (PDF)
type: Document
has file: Thesis_LinaStreich.pdf
has part: Thesis_LinaStreich.pdf

Lina Lou Streich
type: Person
given name: Lina Lou
family name: Streich

Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (Other)
type: Document
is version of: Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (PDF)
is volatile version of: Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (PDF)
is index codes version of: Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (PDF)
has file: indexcodes.txt
has part: indexcodes.txt

Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (Other)
type: Document
is version of: Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (PDF)
is volatile version of: Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (PDF)
islightbox thumbnail version of: Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (PDF)
has file: lightbox.jpg
has part: lightbox.jpg

Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (Other)
type: Document
is version of: Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (PDF)
is volatile version of: Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (PDF)
ispreview thumbnail version of: Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (PDF)
has file: preview.jpg
has part: preview.jpg

Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (Other)
type: Document
is version of: Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (PDF)
is volatile version of: Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (PDF)
ismedium thumbnail version of: Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (PDF)
has file: medium.jpg
has part: medium.jpg

Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (Other)
type: Document
is version of: Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (PDF)
is volatile version of: Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (PDF)
issmall thumbnail version of: Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse (PDF)
has file: small.jpg
has part: small.jpg

HTML Summary of #30689 Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse
format: text/html
primary topic: Development of adaptive-optics and multi-photon microscopy techniques for high-resolution deep-tissue imaging in the in-vivo mouse

000 Allgemeines, Wissenschaft, Informatik
type: Concept
preferred label: 000 Generalities, Science @en

500 Naturwissenschaften und Mathematik
type: Concept
preferred label: 500 Natural sciences and mathematics @en

530 Physik
type: Concept
preferred label: 530 Physics @en

570 Biowissenschaften, Biologie
type: Concept
preferred label: 570 Life sciences @en