%0 Generic
%A Knoch, Tobias Aurelius
%D 2002
%F heidok:3105
%K Genom Organisation, Chromosomen Struktur, lang-reichweitige Korrelationen, in vivo Chromatin Markierung, simultane Ko-Transfektiongenome organisation, chromosome structure, long-range correlation, in vivo chromatin labelling, simultaneous co-transfection
%R 10.11588/heidok.00003105
%T Approaching the Three-Dimensional Organization of the Human Genome
%U https://archiv.ub.uni-heidelberg.de/volltextserver/3105/
%X To approach the still largely unknown sequential and three-dimensional organization of the human cell nucleus, the structural-, scaling- and dynamic properties of interphase chromosomes and cell nuclei were simulated on the 30 nm chromatin fiber level with Monte Carlo, Brownian Dynamics and parallel computing methods. Differences between used models explain various experimental conditions, favouring a Multi-Loop-Subcompartment model with 63-126 kbp loops aggregated to possibly rosettes connected by 63-126 kbp linkers, and predict correctly the transport of molecules by moderately obstructed diffusion excluding the Inter-Chromosomal Domain hypothesis. Correlation analyses of completely sequenced Archaea, Bacteria and Eukarya chromosomes revealed fine-structured positive long-range correlations due to codon, nucleosomal or block organization of the genomes, allowing classification and tree construction. By construction and expression of fusionproteins from the histones H1, H2A, H2B, H3, H4 and mH2A1.2 with the autofluorescent proteins CFP, GFP, YFP, DsRed-1 and DsRed-2, the chromatin morphology could be investigated in vivo during interphase, mitosis or apoptosis and revealed different interphase morphologies for cell lines, quantifiable by scaling analyses. Finally, construct conversions in simultaneous co-transfections due to recombination/repair/replication were discovered in *25 % of cells and led to a variety of new applications.
%Z Teile in: Knoch, T. A. Approaching the three-dimensional organization of the human genome. TAK Press, Dr. Tobias A. Knoch, Mannheim, Germany, ISBN 3-00-009959-X (soft cover, 3rd ed.), ISBN 3-00-009960-3 (hard cover, 3rd ed.), 2002.