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primary topic: The Origin and Regulation of Segmentation Clock Oscillation Dynamics in the Mouse Embryo

The Origin and Regulation of Segmentation Clock Oscillation Dynamics in the Mouse Embryo
type: Article
abstract: How cells coordinate their dynamic gene expression to produce patterns that transcend to the tissue scale is a fundamental question in biology. In the context of somitogenesis, the timing of segmentation is controlled by a molecular oscillator, the segmentation clock. The segmentation clock oscillations, particularly those regulated by Notch signaling, exhibit traveling wave patterns in the presomitic mesoderm (PSM) due to phase shifted oscillations along the AP axis. How their spatiotemporal dynamics are coordinated to produce such patterns is not fully understood. I first describe the origin of the traveling waves, with data obtained by imaging the onset of segmentation clock dynamics, at the gastrulating stages of the mouse embryo. Detailed analysis of the oscillations revealed that they are initially synchronous across space at its onset, but accumulate spatial phase shift over the first 6+ cycles, thereby leading to the emergence of traveling wave patterns. Such an accumulation of spatial phase shift agrees with the presence of a period gradient, where period mismatches between adjacent tissue increase the lag in oscillation phase over time. The existence of an oscillation period gradient has been reported in the later stages of the embryo across species, but not much is known about its regulation. FGF and Wnt signaling gradients present in the PSM have been nominated to control oscillation dynamics. However, past studies have reported that FGF signaling does not affect the segmentation clock, as segmentation pace was unaltered when FGF signaling was perturbed. Using an in vitro model of the PSM also showing an emergence of traveling waves, I characterized the effect of exogenous FGF signal on the wave dynamics in detail. The series of spatially and temporally designed FGF addition experiments indicate that FGF signaling governs the rate of change in oscillation period, or biologically, the rate of cellular maturation towards differentiation. Taken together, I …
date: 2022
author list: authors
type: EPrint
type: DoctoralThesisEPrint
is part of: repository
has document: The Origin and Regulation of Segmentation Clock Oscillation Dynamics in the Mouse Embryo (PDF)
has document: The Origin and Regulation of Segmentation Clock Oscillation Dynamics in the Mouse Embryo (Other)
subject: 500 Naturwissenschaften und Mathematik
subject: 570 Biowissenschaften, Biologie

The Origin and Regulation of Segmentation Clock Oscillation Dynamics in the Mouse Embryo (PDF)
type: Document
has file: Thesis_TT_2021.pdf
has part: Thesis_TT_2021.pdf

Takehito Tomita
type: Person
given name: Takehito
family name: Tomita

The Origin and Regulation of Segmentation Clock Oscillation Dynamics in the Mouse Embryo (Other)
type: Document
is version of: The Origin and Regulation of Segmentation Clock Oscillation Dynamics in the Mouse Embryo (PDF)
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The Origin and Regulation of Segmentation Clock Oscillation Dynamics in the Mouse Embryo (Other)
type: Document
is version of: The Origin and Regulation of Segmentation Clock Oscillation Dynamics in the Mouse Embryo (PDF)
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The Origin and Regulation of Segmentation Clock Oscillation Dynamics in the Mouse Embryo (Other)
type: Document
is version of: The Origin and Regulation of Segmentation Clock Oscillation Dynamics in the Mouse Embryo (PDF)
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The Origin and Regulation of Segmentation Clock Oscillation Dynamics in the Mouse Embryo (Other)
type: Document
is version of: The Origin and Regulation of Segmentation Clock Oscillation Dynamics in the Mouse Embryo (PDF)
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The Origin and Regulation of Segmentation Clock Oscillation Dynamics in the Mouse Embryo (Other)
type: Document
is version of: The Origin and Regulation of Segmentation Clock Oscillation Dynamics in the Mouse Embryo (PDF)
is volatile version of: The Origin and Regulation of Segmentation Clock Oscillation Dynamics in the Mouse Embryo (PDF)
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primary topic: The Origin and Regulation of Segmentation Clock Oscillation Dynamics in the Mouse Embryo

500 Naturwissenschaften und Mathematik
type: Concept
preferred label: 500 Natural sciences and mathematics @en

570 Biowissenschaften, Biologie
type: Concept
preferred label: 570 Life sciences @en