<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Endoplasmic reticulum size and shape control in Saccharomyces cerevisiae"^^ . "Cells resize and reshape their organelles in response to changing physiological demands. \r\nThis thesis focuses on the endoplasmic reticulum (ER), an organelle which, depending on cell \r\ntype and physiological conditions, displays remarkable adaptability in both size and shape. \r\nAlthough much is known about the role of proteins in ER morphogenesis, the mechanisms \r\ncontrolling the biogenesis of its membrane, and hence size, are unclear. In this work, I build \r\nupon a genetic screen performed to identify genes involved in ER expansion in the budding \r\nyeast, Saccharomyces cerevisiae, and I focus on the poorly characterised ER membrane \r\nprotein Ice2p. I show that Ice2p is required for and promotes ER expansion, and that it does \r\nso independently of the central determinant of ER homeostasis, the unfolded protein \r\nresponse (UPR). To further uncover the molecular role of Ice2p, I explore known genetic \r\ninteractions and discover that Ice2p opposes the activity of Pah1p, which is a conserved \r\nphosphatidic acid phosphatase with a central role in the regulation of lipid synthesis in yeast. \r\nSpecifically, I show that Ice2p interacts with and inhibits the conserved Spo7p-Nem1p \r\ncomplex, which normally dephosphorylates and activates Pah1p. By showing that Ice2p \r\ncooperates with pathways transcriptionally controlling lipid synthesis, and with the UPR to \r\nmaintain cell homeostasis, I place Ice2 into a broader cellular context. Additionally, I present \r\npreliminary data approaching the physiological conditions during which cells use Ice2p-Pah1p to control the size of their ER. Finally, in the last part of the thesis, I follow up on the \r\nunexpected observation that the reticulon proteins, which are membrane proteins with a \r\ncentral and conserved role in ER morphogenesis, form cytosolic puncta after prolonged ER \r\nstress, a phenotype especially pronounced in cells lacking Ice2p. These data suggest that \r\nthere are mechanisms controlling the membrane association of reticulon proteins, and as an \r\nextension ER shape. Overall, this thesis extends the understanding of mechanisms regulating \r\nthe biogenesis of ER membrane as well as its morphogenesis and provides the ground for \r\nfuture work not only in yeast but also in higher eukaryotes."^^ . "2022" . . . . . . . "Dimitrios"^^ . "Papagiannidis"^^ . "Dimitrios Papagiannidis"^^ . . . . . . "Endoplasmic reticulum size and shape control in Saccharomyces cerevisiae (PDF)"^^ . . . "Papagiannidis_PhD_thesis.pdf"^^ . . . "Endoplasmic reticulum size and shape control in Saccharomyces cerevisiae (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Endoplasmic reticulum size and shape control in Saccharomyces cerevisiae (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Endoplasmic reticulum size and shape control in Saccharomyces cerevisiae (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Endoplasmic reticulum size and shape control in Saccharomyces cerevisiae (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Endoplasmic reticulum size and shape control in Saccharomyces cerevisiae (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #31712 \n\nEndoplasmic reticulum size and shape control in Saccharomyces cerevisiae\n\n" . "text/html" . . . "500 Naturwissenschaften und Mathematik"@de . "500 Natural sciences and mathematics"@en . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .