title: A rapid and simple procedure for the isolation and cultivation of fibroblast-like cells from medaka and zebrafish embryos and fin clip biopsies
creator: Beedgen, Lars
creator: Hüllen, Andreas
creator: Gücüm, Sevinç
creator: Thumberger, Thomas
creator: Wittbrodt, Jochen
creator: Thiel, Christian
subject: ddc-610
subject: 610 Medical sciences Medicine
description: In many human diseases, the molecular pathophysiological mechanisms are not understood, which makes the development and testing of new therapeutic approaches difficult. The generation and characterization of animal models such as mice, rats, fruit flies, worms or fish offers the possibility for in detail studies of a disease’s development, its course and potential therapies in an organismal context, which considerably minimizes the risk of therapeutic side effects for patients. Nevertheless, due to the high numbers of experimental animals used in research worldwide, attempts to develop alternative test systems will help in reducing their count. In this regard, the cell culture system displays a suitable option due to its potential of delivering nearly unlimited material and the good opportunities for high-throughput studies such as drug testing. Here, we describe a quick and simple method to isolate and cultivate vital fibroblast-like cells from embryos and adults of two popular teleost model organisms, the Japanese rice fish medaka (Oryzias latipes) and the zebrafish (Danio rerio).
publisher: Sage Publications
date: 2022
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/31793/1/10.1177_00236772211045483.pdf
identifier: DOI:10.11588/heidok.00031793
identifier: https://doi.org/10.1177/00236772211045483
identifier: urn:nbn:de:bsz:16-heidok-317939
identifier:   Beedgen, Lars ; Hüllen, Andreas ; Gücüm, Sevinç ; Thumberger, Thomas ; Wittbrodt, Jochen ; Thiel, Christian  (2022) A rapid and simple procedure for the isolation and cultivation of fibroblast-like cells from medaka and zebrafish embryos and fin clip biopsies.  Laboratory Animals, 56 (3).  pp. 270-278.  ISSN 0023-6772 (Druck-Ausg.); 1758-1117 (Online-Ausg.)     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/31793/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng