TY  - JOUR
VL  - 56
CY  - London
KW  - Fibroblast-like cells
KW  -  fish cell culture
KW  -  fibroblasts
KW  -  medaka
KW  -  zebrafish
KW  -  embryo
KW  -  fin clip
EP  - 278
IS  - 3
A1  - Beedgen, Lars
A1  - Hüllen, Andreas
A1  - Gücüm, Sevinç
A1  - Thumberger, Thomas
A1  - Wittbrodt, Jochen
A1  - Thiel, Christian
N2  - In many human diseases, the molecular pathophysiological mechanisms are not understood, which makes the development and testing of new therapeutic approaches difficult. The generation and characterization of animal models such as mice, rats, fruit flies, worms or fish offers the possibility for in detail studies of a disease?s development, its course and potential therapies in an organismal context, which considerably?minimizes the risk of therapeutic side effects for patients. Nevertheless, due to the high numbers of experimental animals used in research worldwide, attempts to develop alternative test systems will help in reducing their count. In this regard, the cell culture system displays a suitable option due to its potential of delivering nearly unlimited material and the good opportunities for high-throughput studies such as drug testing. Here, we describe a quick and simple method to isolate and cultivate vital fibroblast-like cells from embryos and adults of two popular teleost model organisms, the Japanese rice fish medaka (Oryzias latipes) and the zebrafish (Danio rerio).
N1  - Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz bzw. Nationallizenz frei zugänglich. *** This publication is freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
Y1  - 2022///
TI  - A rapid and simple procedure for the isolation and cultivation of fibroblast-like cells from medaka and zebrafish embryos and fin clip biopsies
AV  - public
SP  - 270
JF  - Laboratory Animals
ID  - heidok31793
UR  - https://doi.org/10.1177/00236772211045483
SN  - 0023-6772 (Druck-Ausg.); 1758-1117 (Online-Ausg.)
PB  - Sage Publications
ER  -