eprintid: 31793
rev_number: 16
eprint_status: archive
userid: 5878
dir: disk0/00/03/17/93
datestamp: 2022-06-21 12:19:27
lastmod: 2022-10-06 07:02:50
status_changed: 2022-06-21 12:19:27
type: article
metadata_visibility: show
creators_name: Beedgen, Lars
creators_name: Hüllen, Andreas
creators_name: Gücüm, Sevinç
creators_name: Thumberger, Thomas
creators_name: Wittbrodt, Jochen
creators_name: Thiel, Christian
title: A rapid and simple procedure for the isolation and cultivation of fibroblast-like cells from medaka and zebrafish embryos and fin clip biopsies
subjects: ddc-610
divisions: i-721000
divisions: i-741000
divisions: i-741020
divisions: i-910500
keywords: Fibroblast-like cells, fish cell culture, fibroblasts, medaka, zebrafish, embryo, fin clip
note: Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz bzw. Nationallizenz frei zugänglich. *** This publication is freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
abstract: In many human diseases, the molecular pathophysiological mechanisms are not understood, which makes the development and testing of new therapeutic approaches difficult. The generation and characterization of animal models such as mice, rats, fruit flies, worms or fish offers the possibility for in detail studies of a disease’s development, its course and potential therapies in an organismal context, which considerably minimizes the risk of therapeutic side effects for patients. Nevertheless, due to the high numbers of experimental animals used in research worldwide, attempts to develop alternative test systems will help in reducing their count. In this regard, the cell culture system displays a suitable option due to its potential of delivering nearly unlimited material and the good opportunities for high-throughput studies such as drug testing. Here, we describe a quick and simple method to isolate and cultivate vital fibroblast-like cells from embryos and adults of two popular teleost model organisms, the Japanese rice fish medaka (Oryzias latipes) and the zebrafish (Danio rerio).
date: 2022
publisher: Sage Publications
id_scheme: DOI
id_number: 10.11588/heidok.00031793
official_url: https://doi.org/10.1177/00236772211045483
ppn_swb: 181810296X
own_urn: urn:nbn:de:bsz:16-heidok-317939
language: eng
bibsort: BEEDGENLARARAPIDANDS2022
full_text_status: public
publication: Laboratory Animals
volume: 56
number: 3
place_of_pub: London
pagerange: 270-278
issn: 0023-6772 (Druck-Ausg.); 1758-1117 (Online-Ausg.)
edition: Zweitveröffentlichung
citation:   Beedgen, Lars ; Hüllen, Andreas ; Gücüm, Sevinç ; Thumberger, Thomas ; Wittbrodt, Jochen ; Thiel, Christian  (2022) A rapid and simple procedure for the isolation and cultivation of fibroblast-like cells from medaka and zebrafish embryos and fin clip biopsies.  Laboratory Animals, 56 (3).  pp. 270-278.  ISSN 0023-6772 (Druck-Ausg.); 1758-1117 (Online-Ausg.)     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/31793/1/10.1177_00236772211045483.pdf