<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "The role of membrane tethering complexes in vacuolar trafficking and vacuole biogenesis"^^ . "The lytic vacuole is vital for the plant cell, as the vacuole fulfills essential functions, such as degradation, detoxification, storage and generation of the turgor pressure. Secondary active transport and degradation processes require the energy provided by the tonoplast-localized V-ATPase and V-PPase. By studying the vacuolar trafficking of the V-ATPase and the V-PPase a trafficking route independent of the Golgi and mediated by provacuoles was discovered. To further investigate the Golgi-independent trafficking and its role in vacuole biogenesis, conditional expression of Sar1b-GTP to disrupt COPII-mediated trafficking was studied. The tonoplast-localized V-ATPase can be transported independent of the COPII route to the vacuole. With a genetic piggyback system and conditional expression of VHA-a3 punctate and tubular structures likely being provacuoles were examined in vivo. \r\nThe role of the conserved membrane tethering complexes HOPS and CORVET in vacuolar trafficking was investigated. In Arabidopsis, the HOPS complex mediates vacuolar trafficking from the Golgi via AP-3 vesicles and from the TGN/EE via MVBs, similar to its function in yeast and mammalian cells. CORVET evolved a new role and operates a plant-specific transport route from the TGN/EE to the vacuole. A population of the tonoplast-localized V-ATPase and V-PPase enters a Golgi-dependent pathway, which passes through the TGN/EE and RAB5-positive LEs, which require the CORVET membrane tethering complex for fusion with the vacuole. However, neither the HOPS nor the CORVET complex seems to play a role in membrane fusion between provacuoles and the vacuole. Tonoplast-localized proton pumps can be sorted into provacuoles as well as into COPII vesicles. Thereby, tonoplast-localized proton pumps can exit the ER via two distinct routes and the distribution between the two trafficking routes might be adaptable to environmental or developmental cues.\r\nIn meristematic root cells, the vacuole is composed of a single, tubular network, whose integrity is maintained by the HOPS complex. Homotypic vacuole fusion occurs via the formation of vertex rings. Unlike in yeast cells, the V-ATPase is not required for homotypic vacuole fusion. However, the balance of the signaling lipid PI3P at the vacuolar membrane is crucial for proper fusion events at the vacuole."^^ . "2022" . . . . . . . "Jana Christin"^^ . "Askani"^^ . "Jana Christin Askani"^^ . . . . . . "The role of membrane tethering complexes in vacuolar trafficking and vacuole biogenesis (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "The role of membrane tethering complexes in vacuolar trafficking and vacuole biogenesis (PDF)"^^ . . . "Dissertation_JanaAskani_heiDOK.pdf"^^ . . . "The role of membrane tethering complexes in vacuolar trafficking and vacuole biogenesis (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "The role of membrane tethering complexes in vacuolar trafficking and vacuole biogenesis (Other)"^^ . . . . . . "preview.jpg"^^ . . . "The role of membrane tethering complexes in vacuolar trafficking and vacuole biogenesis (Other)"^^ . . . . . . "medium.jpg"^^ . . . "The role of membrane tethering complexes in vacuolar trafficking and vacuole biogenesis (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #32152 \n\nThe role of membrane tethering complexes in vacuolar trafficking and vacuole biogenesis\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .