<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Metabolic, proteomic, transcriptomic, and fatty acid profiling of glioblastoma stem cells and their small extracellular vesicles"^^ . "Glioblastoma is the most frequent and lethal brain tumor, showing a high degree of intra- and intertumoral\r\nheterogeneity. Despite the multimodal treatments (surgical resection, irradiation, and chemotherapy),\r\noverall survival of patients is still less than 15 months. The inevitable relapse of glioblastoma is mainly\r\nattributed to the subpopulation of tumor initiating cells, namely glioblastoma stem cells, which are shown\r\nto be refractory to standard treatments. The exceedingly heterogeneous nature of glioblastoma creates\r\nhurdles in developing effective therapies and makes it extremely difficult to eradicate. This project\r\ninvestigated the role of GSCs-derived sEVs in glioblastoma aggressiveness with the particular focus on\r\nthe complexity of tumor-derived sEVs in terms of proteins, metabolites, fatty acids, and small RNAs. In\r\nthis direction, protein, metabolite, fatty acid, and small-RNA contents of sEVs and their parent cells\r\n(NCH421k, NCH644, NCH705, and NCH711d) were profiled by utilizing mass spectrometry and highthroughput\r\nsequencing. Protein profiling of sEVs and their respective cell lines revealed the enrichment\r\nof proteins playing a role in amino acids, carboxylic acids, and organic acids transmembrane transport,\r\ntogether with the ones functioning in growth factor binding (including insulin-like growth factor I and\r\ntransforming growth factor beta). In line with the proteomic analysis, metabolite screening of GSCderived\r\nsEVs also displayed the existence of metabolites associated with alanine, aspartate, glutamate\r\nmetabolism, arginine biosynthesis, and butanoate metabolism, suggesting the dual role of GSCs-derived\r\nsEVs, which is transferring proteins responsible for the transport of amino acids/carboxylic acids and\r\nproviding metabolites that will be used in amino acid and carboxylic acid metabolism. In conjunction with\r\nproteomic and metabolic analysis, profiling of fatty acids (carboxylic acids with long aliphatic chains) has\r\nrevealed the presence of different fatty acid species, especially saturated fatty acids, in GSCs-derived\r\nsEVs, further implying that loading of biological cargos into sEVs is a highly regulated process, and that\r\nGSCs-derived sEVs are important sources for tumor cells to maintain their cellular metabolism. Finally,\r\nsmall RNA sequencing of GSCs-derived sEVs and their parent cells was carried out to shed some light\r\non the contribution of sEVs-derived small RNAs to heterogeneity of glioblastoma; however, due to\r\ntechnical problems in sequencing (very low reads counts, poor complexity of sequencing library) the data\r\nis unfortunately not reliable for comparative analysis.\r\nIn summary, this project revealed the complexity of GSCs-derived sEVs in terms of proteins, metabolites,\r\nfatty acids, and smRNAs (with limited degree), and unveiled their potential contribution to tumor\r\nheterogeneity and critical cellular processes commonly deregulated in glioblastoma."^^ . "2023" . . . . . . . "Tolga"^^ . "Lokumcu"^^ . "Tolga Lokumcu"^^ . . . . . . "Metabolic, proteomic, transcriptomic, and fatty acid profiling of glioblastoma stem cells and their small extracellular vesicles (Other)"^^ . . . . . . "small.jpg"^^ . . . "Metabolic, proteomic, transcriptomic, and fatty acid profiling of glioblastoma stem cells and their small extracellular vesicles (PDF)"^^ . . . "PhD Thesis_Tolga Lokumcu_FINAL_VERSION_081121.pdf"^^ . . . "Metabolic, proteomic, transcriptomic, and fatty acid profiling of glioblastoma stem cells and their small extracellular vesicles (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Metabolic, proteomic, transcriptomic, and fatty acid profiling of glioblastoma stem cells and their small extracellular vesicles (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Metabolic, proteomic, transcriptomic, and fatty acid profiling of glioblastoma stem cells and their small extracellular vesicles (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Metabolic, proteomic, transcriptomic, and fatty acid profiling of glioblastoma stem cells and their small extracellular vesicles (Other)"^^ . . . . . . "medium.jpg"^^ . . "HTML Summary of #32720 \n\nMetabolic, proteomic, transcriptomic, and fatty acid profiling of glioblastoma stem cells and their small extracellular vesicles\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . . . "610 Medizin"@de . "610 Medical sciences Medicine"@en . .