<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Coordination Mechanisms of Mammalian\r\nEmbryo Implantation"^^ . "A direct interaction between the extraembryonic and the uterine tissues during embryo implantation generates a unique biomechanical context for the blastocyst. However, our mechanistic understanding of the regulation of blastocyst morphogenesis during implantation is limited by the inaccessibility in vivo and remaining challenges to model feto-maternal interaction ex vivo. To overcome these limitations, I applied microfabrication and biomaterial engineering to model biomechanical cues of the murine intrauterine environment ex vivo with high precision and tunability. I identify that embryo-uterine adhesion and tissue geometry are critical for successful peri-implantation development. In a specific parameter range, closely resembling in utero conditions, the 3D geometrically patterned hydrogel supports mouse blastocysts through implantation and enables robust peri-implantation morphogenesis; promotes the development of the Reichert’s membrane and all extraembryonic tissues, including giant trophoblast, which directly interacts with the uterus.\r\n\r\nTo monitor in toto peri-implantation embryo dynamics, the culture method was\r\nintegrated with inverted view InVi-SPIM and multiview MuVi-SPIM light-sheet\r\nmicroscopes. I show that integrin-mediated adhesion by the mural trophectoderm\r\nprovides the mechanism of trophectoderm tension release, driving the morphogenesis of the extraembryonic ectoderm and egg cylinder patterning. Moreover, the embryo-uterine adhesion enables collective trophoblast migration, dependent on Rac1. Finally, I demonstrate that the uterine tissue geometry spatially coordinates collective trophoblast migration to delineate space for egg cylinder growth. Together, this study reveals essential mechanisms of dynamic embryo-uterus interactions during peri-implantation development."^^ . "2023" . . . . . . . "Vladyslav"^^ . "Bondarenko"^^ . "Vladyslav Bondarenko"^^ . . . . . . "Coordination Mechanisms of Mammalian\r\nEmbryo Implantation (PDF)"^^ . . . "Thesis_VBondarenko.pdf"^^ . . . "Coordination Mechanisms of Mammalian\r\nEmbryo Implantation (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Coordination Mechanisms of Mammalian\r\nEmbryo Implantation (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Coordination Mechanisms of Mammalian\r\nEmbryo Implantation (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Coordination Mechanisms of Mammalian\r\nEmbryo Implantation (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Coordination Mechanisms of Mammalian\r\nEmbryo Implantation (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #33153 \n\nCoordination Mechanisms of Mammalian \nEmbryo Implantation\n\n" . "text/html" . . . "000 Allgemeines, Wissenschaft, Informatik"@de . "000 Generalities, Science"@en . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .