<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "3D-4Pi MINFLUX nanoscopy"^^ . "Determining the position of fluorescent emitters in all three spatial dimensions and the temporal dimension is an essential tool in the life sciences because it allows dynamic processes in biological structures to be studied with high precision and minimal invasiveness. The rate of emitted photons usually limits the achievable spatio-temporal precision of such investigations. In this work, it is shown both theoretically and experimentally that in a\r\nMINFLUX setup with a 4Pi interferometer, this resource can be optimally leveraged by using coherently interfering counterpropagating beams. 3D isotropic precision is achieved by introducing tilts to these beams in the focal region. To enable 3D MINFLUX measurements, a phase scanner and a beam scanner have been developed and implemented in a 4Pi optics. The former allows for shifting an interferometrically generated local minimum of the excitation laser along the propagation axis of the laser beams. The latter allows positioning the laser beam in the pupil of the microscope objectives, tilting the measurement axis. Both scanners are based on crystal optics, so they can be controlled electro-optically with the highest spatial and temporal precision. With the MINFLUX microscope used here, measurements on single molecules are shown to have a 40 % higher photon efficiency than previous 3D MINFLUX measurements. In addition, sub-nm precision at short time scales on bright, photostable emitters at a measurement rate of approximately 9 kHz per axis is demonstrated. The high spatio-temporal precision of the system is used to gain new insights into the stepping behavior of DNA nanorobots and the 3D diffusion of single molecules and fluorescent beads. The results obtained demonstrate the superiority of a 3D-4Pi MINFLUX microscope in tracking single fluorescent emitters in three dimensions."^^ . "2024" . . . . . . . "Julian Dominik"^^ . "Rickert"^^ . "Julian Dominik Rickert"^^ . . . . . . "3D-4Pi MINFLUX nanoscopy (PDF)"^^ . . . "Thesis_pdfa.pdf"^^ . . . "3D-4Pi MINFLUX nanoscopy (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "3D-4Pi MINFLUX nanoscopy (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "3D-4Pi MINFLUX nanoscopy (Other)"^^ . . . . . . "preview.jpg"^^ . . . "3D-4Pi MINFLUX nanoscopy (Other)"^^ . . . . . . "medium.jpg"^^ . . . "3D-4Pi MINFLUX nanoscopy (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #33275 \n\n3D-4Pi MINFLUX nanoscopy\n\n" . "text/html" . . . "530 Physik"@de . "530 Physics"@en . .