<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Mechanical characterisation of biological cells and biofunctional interfaces"^^ . "Biological cells sense the mechanical properties of their surrounding environment and adapt their\r\nshape and function. Moreover, the mechanical properties of cells and tissues tightly correlate with\r\ntheir functions. The main thrust of this thesis is to quantitatively determine the mechanical proper-\r\nties of cells and cell-repellent coating materials by the combination of unique experimental techniques\r\nby covering different spatio-temporal domains.\r\nIn chapter 7 the viscoelastic shape relaxation of malaria-infected human red blood cells with a di-\r\nameter of about 10 μm was monitored by the combination of a custom-designed microfluidic device\r\nand a high-speed imaging platform under collaboration with Prof. Dr. M. Lanzer (Center for Inte-\r\ngrative Infectious Diseases, Heidelberg University). Using the binarised cell rims extracted from the\r\nlive-cell images, the shape recovery of red blood cells upon the ejection from the narrow constriction\r\nwas monitored with a time resolution of 30 μs per frame. The mechanical responses of the malaria-\r\ninfected red blood cells were monitored through the entire life cycle of parasites. The systematic\r\ncomparison of the red blood cells with genetically mutated hemoglobin (hemoglobinopathie) with\r\nnormal red blood cells indicated a less pronounced change in the relaxation time in hemoglobinopa-\r\nthetic red blood cells, which might correlate with delayed protein synthesis in hemoglobinopathetic\r\nred blood cells.\r\nIn chapter 8 the film elastic properties and internal structures of the monolayers of oligoethylene\r\nglycol-based dendrons for the coating of iron-oxide nanoparticles were studied by the combination\r\nof high energy X-ray reflectivity and high-speed atomic force microscopy. To achieve higher film sta-\r\nbility in blood stream, the dendrons, synthesized by the group of Prof. Dr. Felder-Flesch (Institut\r\nde Physique et Chimie des Materiaux , Univ. Strasbourg) were coupled to the oxide surface via two\r\nphosphonate groups. The interfacial force measurements were performed on planar silicon dioxide\r\nsurfaces instead of iron oxide nanoparticle surfaces due to the technical limitations. The internal\r\nstructures of dendron monolayers in water were probed by high energy specular X-ray reflectivity.\r\nAn analytical model considering the transition from a soft layer to a hard layer was introduced to cal-\r\nculate the Young’s modulus from nm-thick monolayers. To gain deeper insights into the interfacial\r\nforce interactions, the coarse-scale surface force-distance curves were measured by a cell-sized particle\r\nattached to an atomic force cantilever cantilever, while the size and distribution of nanoscopic pin-\r\nning centers were monitored by fast force mapping with a pixel rate of 200 Hz. The capability of\r\nthe dendron coating to prevent the platelet aggregation was assessed by observing the non-specific\r\nadhesion of human platelets on dendron-coated substrates. The dynamic uptake and localisation\r\nof fluorescent dendron-coated iron oxide nanoparticles into hypoxic mouse breast cancer cells was\r\ntracked using fluorescence imaging and cryo-transmission electron microscopy. Together, these meth-\r\nods revealed a continuous uptake of iron oxide nanoparticles into in intracellular compartments such\r\nas endosomes via endocytosis. The iron oxide particles were found either agglomerated or as single\r\nnanoparticles."^^ . "2023" . . . . . . . "Julian Angel"^^ . "Czajor"^^ . "Julian Angel Czajor"^^ . . . . . . "Mechanical characterisation of biological cells and biofunctional interfaces (PDF)"^^ . . . "Dissertation_Czajor_Julian_2023.pdf"^^ . . . "Mechanical characterisation of biological cells and biofunctional interfaces (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Mechanical characterisation of biological cells and biofunctional interfaces (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Mechanical characterisation of biological cells and biofunctional interfaces (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Mechanical characterisation of biological cells and biofunctional interfaces (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Mechanical characterisation of biological cells and biofunctional interfaces (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #33449 \n\nMechanical characterisation of biological cells and biofunctional interfaces\n\n" . "text/html" . . . "500 Naturwissenschaften und Mathematik"@de . "500 Natural sciences and mathematics"@en . . . "530 Physik"@de . "530 Physics"@en . . . "540 Chemie"@de . "540 Chemistry and allied sciences"@en . .