eprintid: 33483
rev_number: 13
eprint_status: archive
userid: 7491
dir: disk0/00/03/34/83
datestamp: 2023-07-11 10:59:52
lastmod: 2023-07-11 16:57:41
status_changed: 2023-07-11 10:59:52
type: doctoralThesis
metadata_visibility: show
creators_name: Dreher, Yannik Aaron
title: Engineering Synthetic Cell Division: Towards the Creation of Self-Replicating Cells from the Bottom up
subjects: ddc-620
divisions: i-160001
adv_faculty: af-19
abstract: Bottom-up synthetic biology has set itself the exciting and ambitious task to create cellular life from scratch. In particular, the successful mimicry of cell division, which is widely considered one of its fundamental features, represents a milestone towards this goal. 
However, the complexity of cellular division machineries limits the progress of their reconstitution into synthetic cells, opening the way for novel and creative approaches for the bottom-up engineering of synthetic cell division. \\
In this thesis I engineer mechanisms which can be employed for the implementation and analysis of a synthetic self-replicating cell. In particular, I develop mechanisms for the division of lipid vesicles as synthetic cell compartments with full spatio-temporal control. I present a module which allows for the segregation of DNA droplets as information carriers within synthetic cell compartments. Furthermore, I developed a DNA origami-based genotype-phenotype system which holds important features of natural genotype-phenotpye maps, namely, variability and stability. Additionally, I developed an open-source software tool for 3D object reconstruction and analysis to study structural changes during division and phenotypic effects on vesicle morphology.\\
All in all, these contributions provide valuable strategies for the implementation and analysis of a synthetic self-replicating cell, bringing us closer to the ultimate goal of creating life from scratch.
date: 2023
id_scheme: DOI
id_number: 10.11588/heidok.00033483
ppn_swb: 1852388552
own_urn: urn:nbn:de:bsz:16-heidok-334837
date_accepted: 2023-07-04
advisor: HASH(0x55fc36c00a40)
language: eng
bibsort: DREHERYANNENGINEERIN20230707
full_text_status: public
place_of_pub: Heidelberg
citation:   Dreher, Yannik Aaron  (2023) Engineering Synthetic Cell Division: Towards the Creation of Self-Replicating Cells from the Bottom up.  [Dissertation]     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/33483/1/09052023_Dissertation_YannikDreher.pdf