TY  - GEN
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/33959/
Y1  - 2023///
AV  - public
TI  - Development of axon-carrying dendrite cells in murine hippocampus and primary somatosensory cortex
N2  - In some neurons, axons originate from a basal dendrite, resulting in an axon-carrying dendrite (AcD) branch with unique functional features. Initial observations showed that the AcD has a privileged position, allowing circumvention of somatic inhibition and providing a highly efficient synaptic input channel, resulting in potentially distinct functional implications for overall network activity. Although AcD neurons have been described in numerous anatomical regions across species, their developmental profile and putative ability to remodel their axonal onset throughout their lifetime remains largely unknown. Therefore, this project investigated the early maturation profile of AcD neurons in comparison to nonAcD neurons in the murine ventral hippocampus (vHC) and primary somatosensory (S1) cortex, using a transgenic mouse line (Thy1-GFP-M). Additionally, to investigate large-scale morphological plasticity, I used immunofluorescence and live-cell imaging of neurons from the mouse primary somatosensory cortex in organotypic slice cultures. In a second approach, I employed an established in vivo paradigm to manipulate sensory input to layer V pyramidal cells in the barrel cortex. This study demonstrates that the proportion of AcD neurons peaks in juvenile mice and that Nav1.6 is clustered more distally in the axon initial segment of AcD neurons than in nonAcD neurons. Furthermore, neurons are indeed capable of changing the onset of their axon origin from somatic to dendritic and vice versa within a few days in vitro. Furthermore, the manipulation of sensory input drives large-scale morphological plasticity especially with regard to the axon onset in vivo. Altogether, the study contributes to the understanding of developmental and activity-dependent plasticity of a functionally important variant of mammalian pyramidal neurons.
A1  - Lehmann, Nadja
CY  - Heidelberg
ID  - heidok33959
ER  -